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PubMed:3131322 JSONTXT 36 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-120 Sentence denotes Kinetic analysis of the Ca2+-dependent, membrane-bound, macrophage phospholipase A2 and the effects of arachidonic acid.
T1 0-120 Sentence denotes Kinetic analysis of the Ca2+-dependent, membrane-bound, macrophage phospholipase A2 and the effects of arachidonic acid.
T2 121-349 Sentence denotes The kinetics of the Ca2+-dependent, alkaline pH optimum, membrane-bound phospholipase A2 from the P388D1 macrophage-like cell line were studied using various phosphatidylcholine (PC) and phosphatidylethanolamine (PE) substrates.
T2 121-349 Sentence denotes The kinetics of the Ca2+-dependent, alkaline pH optimum, membrane-bound phospholipase A2 from the P388D1 macrophage-like cell line were studied using various phosphatidylcholine (PC) and phosphatidylethanolamine (PE) substrates.
T3 350-529 Sentence denotes This enzyme exhibits "surface dilution kinetics" toward PC in Triton X-100 mixed micelles, and the "dual phospholipid model" was found to adequately describe its kinetic behavior.
T3 350-529 Sentence denotes This enzyme exhibits "surface dilution kinetics" toward PC in Triton X-100 mixed micelles, and the "dual phospholipid model" was found to adequately describe its kinetic behavior.
T4 530-659 Sentence denotes With substrate in the form of sonicated vesicles, the dual phospholipid model should give rise to Michaelis-Menten type kinetics.
T4 530-659 Sentence denotes With substrate in the form of sonicated vesicles, the dual phospholipid model should give rise to Michaelis-Menten type kinetics.
T5 660-804 Sentence denotes However, the hydrolysis of dipalmitoyl-PC, 1-palmitoyl-2-oleoyl-PC, and 1-stearoyl-2-arachidonoyl-PC vesicles exhibited two distinct activities.
T5 660-804 Sentence denotes However, the hydrolysis of dipalmitoyl-PC, 1-palmitoyl-2-oleoyl-PC, and 1-stearoyl-2-arachidonoyl-PC vesicles exhibited two distinct activities.
T6 805-984 Sentence denotes Below 10 microM, the data appeared to follow Michaelis-Menten behavior, while at higher concentrations, the data could best be fit to a Hill equation with a Hill coefficient of 2.
T6 805-984 Sentence denotes Below 10 microM, the data appeared to follow Michaelis-Menten behavior, while at higher concentrations, the data could best be fit to a Hill equation with a Hill coefficient of 2.
T7 985-1108 Sentence denotes These PCs had Vmax values for the low substrate concentration range of 0.2-0.6 nmol min-1 mg-1 and Km values of 1-2 microM.
T7 985-1108 Sentence denotes These PCs had Vmax values for the low substrate concentration range of 0.2-0.6 nmol min-1 mg-1 and Km values of 1-2 microM.
T8 1109-1205 Sentence denotes At the high substrate concentration range, the Vmax values were between 5 and 7 nmol min-1 mg-1.
T8 1109-1205 Sentence denotes At the high substrate concentration range, the Vmax values were between 5 and 7 nmol min-1 mg-1.
T9 1206-1374 Sentence denotes PC containing unsaturated fatty acids had an apparent Km, determined from the Hill equation, of about 15 microM, while the apparent Km of dipalmitoyl-PC was 0.6 microM.
T9 1206-1374 Sentence denotes PC containing unsaturated fatty acids had an apparent Km, determined from the Hill equation, of about 15 microM, while the apparent Km of dipalmitoyl-PC was 0.6 microM.
T10 1375-1523 Sentence denotes When 70% glycerol was included in the assays, a single Michaelis-Menten curve was obtained for both dipalmitoyl-PC and 1-stearoyl,2-arachidonoyl-PC.
T10 1375-1523 Sentence denotes When 70% glycerol was included in the assays, a single Michaelis-Menten curve was obtained for both dipalmitoyl-PC and 1-stearoyl,2-arachidonoyl-PC.
T11 1524-1723 Sentence denotes Possible explanations for these kinetic results include reconstitution of the membrane-bound phospholipase A2 in the phospholipid vesicle or the enzyme has tow distinct phospholipid binding function.
T11 1524-1723 Sentence denotes Possible explanations for these kinetic results include reconstitution of the membrane-bound phospholipase A2 in the phospholipid vesicle or the enzyme has tow distinct phospholipid binding function.
T12 1724-1912 Sentence denotes The kinetics for both dipalmitoyl-PC and dipalmitoyl-PE hydrolysis in vesicles was very similar, indicating that the enzyme does not greatly prefer one of these head groups over the other.
T12 1724-1912 Sentence denotes The kinetics for both dipalmitoyl-PC and dipalmitoyl-PE hydrolysis in vesicles was very similar, indicating that the enzyme does not greatly prefer one of these head groups over the other.
T13 1913-1991 Sentence denotes The enzyme also showed no preference for arachidonoyl containing phospholipid.
T13 1913-1991 Sentence denotes The enzyme also showed no preference for arachidonoyl containing phospholipid.
T14 1992-2179 Sentence denotes Enzymatic activity toward PC containing saturated fatty acids was linear to about 15% hydrolysis while the hydrolysis of PC containing unsaturated fatty acids was linear to only about 5%.
T14 1992-2179 Sentence denotes Enzymatic activity toward PC containing saturated fatty acids was linear to about 15% hydrolysis while the hydrolysis of PC containing unsaturated fatty acids was linear to only about 5%.
T15 2180-2261 Sentence denotes This loss of linearity was due to inhibition by released unsaturated fatty acids.
T15 2180-2261 Sentence denotes This loss of linearity was due to inhibition by released unsaturated fatty acids.
T16 2262-2370 Sentence denotes Arachidonic acid was found to be a competitive inhibitor of dipalmitoyl PC hydrolysis with a K1 of 5 microM.
T16 2262-2370 Sentence denotes Arachidonic acid was found to be a competitive inhibitor of dipalmitoyl PC hydrolysis with a K1 of 5 microM.
T17 2371-2455 Sentence denotes This tight binding suggests a possible in vivo regulatory role for arachidonic acid.
T17 2371-2455 Sentence denotes This tight binding suggests a possible in vivo regulatory role for arachidonic acid.
T18 2456-2623 Sentence denotes Three compounds of the arachidonic acid cascade, prostaglandin F2 alpha, 6-keto-prostaglandin F1 alpha, and thromboxane B2, showed no inhibition of enzymatic activity.
T18 2456-2623 Sentence denotes Three compounds of the arachidonic acid cascade, prostaglandin F2 alpha, 6-keto-prostaglandin F1 alpha, and thromboxane B2, showed no inhibition of enzymatic activity.