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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-78 Sentence denotes In Vivo Trapping of Proteins Interacting with the Chloroplast CLPC1 Chaperone:
T1 0-78 Sentence denotes In Vivo Trapping of Proteins Interacting with the Chloroplast CLPC1 Chaperone:
TextSentencer_T2 79-113 Sentence denotes Potential Substrates and Adaptors.
T2 79-113 Sentence denotes Potential Substrates and Adaptors.
TextSentencer_T3 114-198 Sentence denotes The chloroplast stromal CLP protease system is essential for growth and development.
T3 114-198 Sentence denotes The chloroplast stromal CLP protease system is essential for growth and development.
TextSentencer_T4 199-342 Sentence denotes It consists of a proteolytic CLP core complex that likely dynamically interacts with oligomeric rings of CLPC1, CLPC2, or CLPD AAA+ chaperones.
T4 199-342 Sentence denotes It consists of a proteolytic CLP core complex that likely dynamically interacts with oligomeric rings of CLPC1, CLPC2, or CLPD AAA+ chaperones.
TextSentencer_T5 343-534 Sentence denotes These ATP-dependent chaperones are predicted to bind and unfold CLP protease substrates, frequently aided by adaptors (recognins), and feed them into the proteolytic CLP core for degradation.
T5 343-534 Sentence denotes These ATP-dependent chaperones are predicted to bind and unfold CLP protease substrates, frequently aided by adaptors (recognins), and feed them into the proteolytic CLP core for degradation.
TextSentencer_T6 535-885 Sentence denotes To identify new substrates and possibly also new adaptors for the chloroplast CLP protease system, we generated an in vivo CLPC1 substrate trap with a C-terminal STREPII affinity tag in Arabidopsis thaliana by mutating critical glutamate residues (E374A and E718A) in the two Walker B domains of CLPC1 required for the hydrolysis of ATP (CLPC1-TRAP).
T6 535-885 Sentence denotes To identify new substrates and possibly also new adaptors for the chloroplast CLP protease system, we generated an in vivo CLPC1 substrate trap with a C-terminal STREPII affinity tag in Arabidopsis thaliana by mutating critical glutamate residues (E374A and E718A) in the two Walker B domains of CLPC1 required for the hydrolysis of ATP (CLPC1-TRAP).
TextSentencer_T7 886-1039 Sentence denotes On the basis of homology to nonplant CLPB/C chaperones, it is predicted that interacting substrates are unable to be released; that is, they are trapped.
T7 886-1039 Sentence denotes On the basis of homology to nonplant CLPB/C chaperones, it is predicted that interacting substrates are unable to be released; that is, they are trapped.
TextSentencer_T8 1040-1223 Sentence denotes When expressed in the wild type, this CLPC1-TRAP induced a dominant visible phenotype, whereas no viable mutants that express CLPC1-TRAP in the clpc1-1 null mutant could be recovered.
T8 1040-1223 Sentence denotes When expressed in the wild type, this CLPC1-TRAP induced a dominant visible phenotype, whereas no viable mutants that express CLPC1-TRAP in the clpc1-1 null mutant could be recovered.
TextSentencer_T9 1224-1397 Sentence denotes Affinity purification of the CLPC1-TRAP resulted in a dozen proteins highly enriched compared with affinity-purified CLPC1 with a C-terminal STREPII affinity tag (CLPC1-WT).
T9 1224-1397 Sentence denotes Affinity purification of the CLPC1-TRAP resulted in a dozen proteins highly enriched compared with affinity-purified CLPC1 with a C-terminal STREPII affinity tag (CLPC1-WT).
TextSentencer_T10 1398-1479 Sentence denotes These enriched proteins likely represent CLP protease substrates or new adaptors.
T10 1398-1479 Sentence denotes These enriched proteins likely represent CLP protease substrates or new adaptors.
TextSentencer_T11 1480-1654 Sentence denotes Several of these trapped proteins overaccumulated in clp mutants or were found as interactors for the adaptor CLPS1, supporting their functional relationship to CLP function.
T11 1480-1654 Sentence denotes Several of these trapped proteins overaccumulated in clp mutants or were found as interactors for the adaptor CLPS1, supporting their functional relationship to CLP function.
TextSentencer_T12 1655-1919 Sentence denotes Importantly, the affinity purification of this CLPC1-TRAP also showed high enrichment of all CLPP, CLPR, and CLPT subunits, indicating the stabilization of the CLPC to CLP core interaction and providing direct support for their physical and functional interaction.
T12 1655-1919 Sentence denotes Importantly, the affinity purification of this CLPC1-TRAP also showed high enrichment of all CLPP, CLPR, and CLPT subunits, indicating the stabilization of the CLPC to CLP core interaction and providing direct support for their physical and functional interaction.