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PubMed:31055697 JSONTXT 36 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-98 Sentence denotes A versatile salt-based method to immobilize glycosaminoglycans and create growth factor gradients.
T1 0-98 Sentence denotes A versatile salt-based method to immobilize glycosaminoglycans and create growth factor gradients.
TextSentencer_T2 99-212 Sentence denotes Glycosaminoglycans (GAGs) are known to play pivotal roles in physiological processes and pathological conditions.
T2 99-212 Sentence denotes Glycosaminoglycans (GAGs) are known to play pivotal roles in physiological processes and pathological conditions.
TextSentencer_T3 213-299 Sentence denotes To study interactions of GAGs with proteins, immobilization of GAGs is often required.
T3 213-299 Sentence denotes To study interactions of GAGs with proteins, immobilization of GAGs is often required.
TextSentencer_T4 300-453 Sentence denotes Current methodologies for immobilization involve modification of GAGs and/or surfaces, which can be time-consuming and may involve specialized equipment.
T4 300-453 Sentence denotes Current methodologies for immobilization involve modification of GAGs and/or surfaces, which can be time-consuming and may involve specialized equipment.
TextSentencer_T5 454-596 Sentence denotes Here, we use an efficient and low-cost method to immobilize GAGs without any (chemical) modification using highly concentrated salt solutions.
T5 454-596 Sentence denotes Here, we use an efficient and low-cost method to immobilize GAGs without any (chemical) modification using highly concentrated salt solutions.
TextSentencer_T6 597-810 Sentence denotes A number of salts from the Hofmeister series were probed for their capacity to immobilize heparin and chondroitin-6-sulfate on microtiter plates applying single chain antibodies against GAGs for detection (ELISA).
T6 597-810 Sentence denotes A number of salts from the Hofmeister series were probed for their capacity to immobilize heparin and chondroitin-6-sulfate on microtiter plates applying single chain antibodies against GAGs for detection (ELISA).
TextSentencer_T7 811-953 Sentence denotes From all salts tested, the cosmotropic salt ammonium sulfate was most efficient, especially at high concentrations (80-100% (v/v) saturation).
T7 811-953 Sentence denotes From all salts tested, the cosmotropic salt ammonium sulfate was most efficient, especially at high concentrations (80-100% (v/v) saturation).
TextSentencer_T8 954-1128 Sentence denotes Immobilized GAGs were bioavailable as judged by their binding of FGF2 and VEGF, and by their susceptibility towards GAG lyases (heparinase I, II and III, chondroitinase ABC).
T8 954-1128 Sentence denotes Immobilized GAGs were bioavailable as judged by their binding of FGF2 and VEGF, and by their susceptibility towards GAG lyases (heparinase I, II and III, chondroitinase ABC).
TextSentencer_T9 1129-1312 Sentence denotes Using 80% (v/v) saturated ammonium sulfate, block and continuous gradients of heparin were established and a gradient of FGF2 was created using a heparin block gradient as a template.
T9 1129-1312 Sentence denotes Using 80% (v/v) saturated ammonium sulfate, block and continuous gradients of heparin were established and a gradient of FGF2 was created using a heparin block gradient as a template.
TextSentencer_T10 1313-1544 Sentence denotes In conclusion, high concentrations of ammonium sulfate are effective for immobilization of GAGs and for the establishment of gradients of both GAGs and GAG-binding molecules, which enables the study to the biological roles of GAGs.
T10 1313-1544 Sentence denotes In conclusion, high concentrations of ammonium sulfate are effective for immobilization of GAGs and for the establishment of gradients of both GAGs and GAG-binding molecules, which enables the study to the biological roles of GAGs.