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PubMed:3036840 JSONTXT 23 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-73 Sentence denotes Purification and characterization of the beta-adrenergic receptor kinase.
T1 0-73 Sentence denotes Purification and characterization of the beta-adrenergic receptor kinase.
T2 74-363 Sentence denotes The beta-adrenergic receptor kinase (beta-ARK) is a recently discovered enzyme which specifically phosphorylates the agonist-occupied form of the beta-adrenergic receptor (beta-AR) as well as the light-bleached form of rhodopsin. beta-ARK is present in a wide variety of mammalian tissues.
T2 74-363 Sentence denotes The beta-adrenergic receptor kinase (beta-ARK) is a recently discovered enzyme which specifically phosphorylates the agonist-occupied form of the beta-adrenergic receptor (beta-AR) as well as the light-bleached form of rhodopsin. beta-ARK is present in a wide variety of mammalian tissues.
T3 364-548 Sentence denotes The kinase can be purified from bovine cerebral cortex to greater than 90% homogeneity by sequential chromatography on Ultrogel AcA34, DEAE-Sephacel, CM-Fractogel, and hydroxylapatite.
T3 364-548 Sentence denotes The kinase can be purified from bovine cerebral cortex to greater than 90% homogeneity by sequential chromatography on Ultrogel AcA34, DEAE-Sephacel, CM-Fractogel, and hydroxylapatite.
T4 549-639 Sentence denotes This results in an approximately 20,000-fold purification with an overall recovery of 12%.
T4 549-639 Sentence denotes This results in an approximately 20,000-fold purification with an overall recovery of 12%.
T5 640-752 Sentence denotes The purified kinase has an Mr approximately 80,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
T5 640-752 Sentence denotes The purified kinase has an Mr approximately 80,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
T6 753-828 Sentence denotes Several findings indicate that this peptide contains the beta-ARK activity.
T6 753-828 Sentence denotes Several findings indicate that this peptide contains the beta-ARK activity.
T7 829-979 Sentence denotes First, on hydroxylapatite chromatography the enzyme activity coelutes with the Mr approximately 80,000 protein as revealed by Coomassie-Blue staining.
T7 829-979 Sentence denotes First, on hydroxylapatite chromatography the enzyme activity coelutes with the Mr approximately 80,000 protein as revealed by Coomassie-Blue staining.
T8 980-1075 Sentence denotes Second, under phosphorylating conditions the Mr approximately 80,000 protein is phosphorylated.
T8 980-1075 Sentence denotes Second, under phosphorylating conditions the Mr approximately 80,000 protein is phosphorylated.
T9 1076-1188 Sentence denotes Finally, the Mr approximately 80,000 protein specifically interacts with reconstituted agonist-occupied beta-AR.
T9 1076-1188 Sentence denotes Finally, the Mr approximately 80,000 protein specifically interacts with reconstituted agonist-occupied beta-AR.
T10 1189-1358 Sentence denotes Kinetic parameters of the enzyme for beta-AR are Km = 0.25 microM and Vmax = 78 nmol/min/mg whereas for rhodopsin the values are Km = 6 microM and Vmax = 72 nmol/min/mg.
T10 1189-1358 Sentence denotes Kinetic parameters of the enzyme for beta-AR are Km = 0.25 microM and Vmax = 78 nmol/min/mg whereas for rhodopsin the values are Km = 6 microM and Vmax = 72 nmol/min/mg.
T11 1359-1468 Sentence denotes The Km value of the enzyme for ATP is approximately 35 microM using either beta-AR or rhodopsin as substrate.
T11 1359-1468 Sentence denotes The Km value of the enzyme for ATP is approximately 35 microM using either beta-AR or rhodopsin as substrate.
T12 1469-1573 Sentence denotes Receptor phosphorylation by beta-ARK is effectively inhibited by Zn2+, digitonin and a variety of salts.
T12 1469-1573 Sentence denotes Receptor phosphorylation by beta-ARK is effectively inhibited by Zn2+, digitonin and a variety of salts.
T13 1574-1686 Sentence denotes The availability of purified beta-ARK should greatly facilitate studies of its role in receptor desensitization.
T13 1574-1686 Sentence denotes The availability of purified beta-ARK should greatly facilitate studies of its role in receptor desensitization.