| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-151 |
Sentence |
denotes |
The human gut microbe Bacteroides thetaiotaomicron encodes the founding member of a novel glycosaminoglycan-degrading polysaccharide lyase family PL29. |
| T2 |
152-282 |
Sentence |
denotes |
Glycosaminoglycans (GAGs) and GAG-degrading enzymes have wide-ranging applications in the medical and biotechnological industries. |
| T3 |
283-430 |
Sentence |
denotes |
The former are also an important nutrient source for select species of the human gut microbiota (HGM), a key player in host-microbial interactions. |
| T4 |
431-590 |
Sentence |
denotes |
How GAGs are metabolized by the HGM is therefore of interest and has been extensively investigated in the model human gut microbe Bacteroides thetaiotaomicron. |
| T5 |
591-758 |
Sentence |
denotes |
The presence of as-yet uncharacterized GAG-inducible genes in its genome and of related species, however, is testament to our incomplete understanding of this process. |
| T6 |
759-863 |
Sentence |
denotes |
Nevertheless, it presents a potential opportunity for the discovery of additional GAG-degrading enzymes. |
| T7 |
864-1259 |
Sentence |
denotes |
Here, we investigated a gene of unknown function (BT_3328) from the chondroitin sulfate (CS) utilization locus of B. thetaiotaomicron NMR and UV spectroscopic assays revealed that it encodes a novel polysaccharide lyase (PL), hereafter referred to as BtCDH, reflecting its source (B. thetaiotaomicron (Bt)) and its ability to degrade the GAGs CS, dermatan sulfate (DS), and hyaluronic acid (HA). |
| T8 |
1260-1504 |
Sentence |
denotes |
When incubated with HA, BtCDH generated a series of unsaturated HA sugars, including Δ4,5UA-GlcNAc, Δ4,5UA-GlcNAc-GlcA-GlcNac, Δ4,5UA-[GlcNAc-GlcA]2-GlcNac, and Δ4,5UA-[GlcNAc-GlcA]3-GlcNac, as end products and hence was classed as endo-acting. |
| T9 |
1505-1673 |
Sentence |
denotes |
A combination of genetic and biochemical assays revealed that BtCDH localizes to the cell surface of B. thetaiotaomicron where it enables extracellular GAG degradation. |
| T10 |
1674-1846 |
Sentence |
denotes |
BtCDH homologs were also detected in several other HGM species, and we therefore propose that it represents the founding member of a new polysaccharide lyase family (PL29). |
| T11 |
1847-1929 |
Sentence |
denotes |
The current discovery also contributes new insights into CS metabolism by the HGM. |
