| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-128 |
Sentence |
denotes |
Serum responsive proteome reveals correlation between oxidative phosphorylation and morphogenesis in Candida albicans ATCC10231. |
| T2 |
129-315 |
Sentence |
denotes |
To understand the impact of fetal bovine serum (FBS) on metabolism and cellular architecture in addition to morphogenesis, we have identified FBS responsive proteome of Candida albicans. |
| T3 |
316-410 |
Sentence |
denotes |
FBS induced 34% hyphae and 60% pseudohyphae in C. albicans at 30 °C while 98% hyphae at 37 °C. |
| T4 |
411-518 |
Sentence |
denotes |
LC-MS/MS analysis revealed that 285 proteins modulated significantly in response to FBS at 30 °C and 37 °C. |
| T5 |
519-640 |
Sentence |
denotes |
Out of which 152 were upregulated and 62 were downregulated at 30 °C while 18 were up and 53 were downregulated at 37 °C. |
| T6 |
641-886 |
Sentence |
denotes |
Functional annotation suggests that FBS may inhibit glycolysis and fermentative pathway and enhance oxidative phosphorylation (OxPhos), TCA cycle, amino acid and fatty acid metabolism indicating a use of alternative energy source by C. albicans. |
| T7 |
887-1040 |
Sentence |
denotes |
OxPhos inhibition assay using sodium azide corroborated the correlation between inhibition of glycolysis and enhanced OxPhos with pseudohyphae formation. |
| T8 |
1041-1214 |
Sentence |
denotes |
C. albicans induced hyphae in response to FBS irrespective of down regulation of Ras1,Asr1/Asr2, indicates the possible involvement of MAPK and cAMP-PKA independent pathway. |
| T9 |
1215-1392 |
Sentence |
denotes |
The Cell wall of cells grown in presence of FBS at 30 °C was rich in mannan, Beta 1,3-glucan and chitin while membranes were rich in ergosterol compared to those grown at 37 °C. |
| T10 |
1393-1419 |
Sentence |
denotes |
SIGNIFICANCE OF THE STUDY: |
| T11 |
1420-1551 |
Sentence |
denotes |
This is the first study suggesting a correlation between OxPhos and morphogenesis especially pseudohyphae formation in C. albicans. |
| T12 |
1552-1699 |
Sentence |
denotes |
Our data also indicate that fetal bovine serum (FBS) induced morphogenesis is multifactorial and may involve MAPK and cAMP-PKA independent pathway. |
| T13 |
1700-1855 |
Sentence |
denotes |
In addition to morphogenesis, our study provides an insight in to the modulation of metabolism and cellular architecture of C. albicans in response to FBS. |
