> top > projects > sentences > docs > PubMed:28577071 > annotations
sentences Home  

PubMed:28577071 JSONTXT 44 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-95 Sentence denotes Isolation of a methylated mannose-binding protein from terrestrial worm Enchytraeus japonensis.
T1 0-95 Sentence denotes Isolation of a methylated mannose-binding protein from terrestrial worm Enchytraeus japonensis.
TextSentencer_T2 96-390 Sentence denotes To elucidate a biological role of the methylated mannose residues found in N-glycans of terrestrial worm Enchytraeus japonensis, we first synthesized 3-O-methyl mannose- and 4-O-methyl mannose-derivatives and immobilized them to Sepharose 4B beads in order to isolate the sugar-binding protein.
T2 96-390 Sentence denotes To elucidate a biological role of the methylated mannose residues found in N-glycans of terrestrial worm Enchytraeus japonensis, we first synthesized 3-O-methyl mannose- and 4-O-methyl mannose-derivatives and immobilized them to Sepharose 4B beads in order to isolate the sugar-binding protein.
TextSentencer_T3 391-834 Sentence denotes When whole protein extracts from the worms was applied to a series of the columns immobilized with the modified and unmodified mannose-derivatives, respectively, a protein with a molecular weight of 25,000 was isolated by 4-O-methyl mannose-immobilized column chromatography, and termed as a methylated mannose-binding protein (mMBP). mMBP bound weakly to a mannose-immobilized column and moderately to a 3-O-methyl mannose-immobilized column.
T3 391-834 Sentence denotes When whole protein extracts from the worms was applied to a series of the columns immobilized with the modified and unmodified mannose-derivatives, respectively, a protein with a molecular weight of 25,000 was isolated by 4-O-methyl mannose-immobilized column chromatography, and termed as a methylated mannose-binding protein (mMBP). mMBP bound weakly to a mannose-immobilized column and moderately to a 3-O-methyl mannose-immobilized column.
TextSentencer_T4 835-934 Sentence denotes The N-terminal amino acid sequences of mMBP and its endoprotease-digested peptides were determined.
T4 835-934 Sentence denotes The N-terminal amino acid sequences of mMBP and its endoprotease-digested peptides were determined.
TextSentencer_T5 935-1049 Sentence denotes Using the degenerate first primers synthesized based on the primary sequence, a genomic DNA fragment was isolated.
T5 935-1049 Sentence denotes Using the degenerate first primers synthesized based on the primary sequence, a genomic DNA fragment was isolated.
TextSentencer_T6 1050-1212 Sentence denotes Then, the second primers were synthesized based on the genomic DNA fragment, and with use of them two cDNA fragments were obtained by the 3'- and 5'-RACE methods.
T6 1050-1212 Sentence denotes Then, the second primers were synthesized based on the genomic DNA fragment, and with use of them two cDNA fragments were obtained by the 3'- and 5'-RACE methods.
TextSentencer_T7 1213-1489 Sentence denotes Finally, the third primers were synthesized based on the sequences of the two cDNA fragments and one genomic DNA fragment, and with use of them a full-length cDNA of mMBP was isolated and shown to comprise a putative 633 bp open reading frame encoding 210 amino acid residues.
T7 1213-1489 Sentence denotes Finally, the third primers were synthesized based on the sequences of the two cDNA fragments and one genomic DNA fragment, and with use of them a full-length cDNA of mMBP was isolated and shown to comprise a putative 633 bp open reading frame encoding 210 amino acid residues.
TextSentencer_T8 1490-1642 Sentence denotes BLAST analysis revealed that mMBP has identities by 26 ~ 55% to several proteins including the regeneration-upregulated protein 3 from the same species.
T8 1490-1642 Sentence denotes BLAST analysis revealed that mMBP has identities by 26 ~ 55% to several proteins including the regeneration-upregulated protein 3 from the same species.
TextSentencer_T9 1643-1723 Sentence denotes Whether mMBP is involved in the regeneration of the worm is under investigation.
T9 1643-1723 Sentence denotes Whether mMBP is involved in the regeneration of the worm is under investigation.