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Id Subject Object Predicate Lexical cue
T1 0-54 Sentence denotes Purification and characterization of rhodopsin kinase.
T1 0-54 Sentence denotes Purification and characterization of rhodopsin kinase.
T2 55-237 Sentence denotes Rhodopsin kinase was purified to near homogeneity by affinity binding to light-exposed rod cell outer segment membranes, followed by DEAE-cellulose and hydroxyapatite chromatography.
T2 55-237 Sentence denotes Rhodopsin kinase was purified to near homogeneity by affinity binding to light-exposed rod cell outer segment membranes, followed by DEAE-cellulose and hydroxyapatite chromatography.
T3 238-346 Sentence denotes This resulted in a 1055-fold purification of highly active rhodopsin kinase with an overall recovery of 19%.
T3 238-346 Sentence denotes This resulted in a 1055-fold purification of highly active rhodopsin kinase with an overall recovery of 19%.
T4 347-463 Sentence denotes Rhodopsin kinase is a single polypeptide chain with Mr = 67,000-70,000 as determined by gel filtration and SDS-PAGE.
T4 347-463 Sentence denotes Rhodopsin kinase is a single polypeptide chain with Mr = 67,000-70,000 as determined by gel filtration and SDS-PAGE.
T5 464-799 Sentence denotes The kinetic parameters of the enzyme for freshly bleached rhodopsin are Km = 4 microM and Vmax = 700 nmol/min/mg whereas for ATP Km = 2 microM (which is a low value for kinases generally, and about 20 times lower than comparable measurements for a kinase of a similar type, the beta-adrenergic-receptor kinase (Benovic, J.L., Mayor, F.
T5 464-799 Sentence denotes The kinetic parameters of the enzyme for freshly bleached rhodopsin are Km = 4 microM and Vmax = 700 nmol/min/mg whereas for ATP Km = 2 microM (which is a low value for kinases generally, and about 20 times lower than comparable measurements for a kinase of a similar type, the beta-adrenergic-receptor kinase (Benovic, J.L., Mayor, F.
T6 800-865 Sentence denotes Jr., Staniszewski, C., Lefkowitz, R.J., and Caron, M.G. (1987) J.
T6 800-865 Sentence denotes Jr., Staniszewski, C., Lefkowitz, R.J., and Caron, M.G. (1987) J.
T7 866-871 Sentence denotes Biol.
T7 866-871 Sentence denotes Biol.
T8 872-877 Sentence denotes Chem.
T8 872-877 Sentence denotes Chem.
T9 878-894 Sentence denotes 262, 9026-9032).
T9 878-894 Sentence denotes 262, 9026-9032).
T10 895-979 Sentence denotes GTP, on the other hand, is a very poor substrate (Km = 1 mM, Vmax = 10 nmol/min/mg).
T10 895-979 Sentence denotes GTP, on the other hand, is a very poor substrate (Km = 1 mM, Vmax = 10 nmol/min/mg).
T11 980-1124 Sentence denotes Rhodopsin kinase is competitively inhibited by adenosine and its mono- and diphosphate derivatives, but not by most other adenosine derivatives.
T11 980-1124 Sentence denotes Rhodopsin kinase is competitively inhibited by adenosine and its mono- and diphosphate derivatives, but not by most other adenosine derivatives.
T12 1125-1293 Sentence denotes Based upon measurements with 28 nucleotide derivatives, the ATP-binding site of rhodopsin kinase appears to have more specific requirements than that for other kinases.
T12 1125-1293 Sentence denotes Based upon measurements with 28 nucleotide derivatives, the ATP-binding site of rhodopsin kinase appears to have more specific requirements than that for other kinases.
T13 1294-1579 Sentence denotes Compounds such as cGMP, inositol trisphosphate, and others that change concentration during exposure of rod cells to light have only minor inhibitory effects on the kinase activity, with the exception of inositol monophosphate, which can activate the kinase about 20% at 50-100 microM.
T13 1294-1579 Sentence denotes Compounds such as cGMP, inositol trisphosphate, and others that change concentration during exposure of rod cells to light have only minor inhibitory effects on the kinase activity, with the exception of inositol monophosphate, which can activate the kinase about 20% at 50-100 microM.
T14 1580-1724 Sentence denotes Rhodopsin kinase has been difficult to store with retention of activity, but can be successfully stored frozen at -20 degrees C in 20% adonitol.
T14 1580-1724 Sentence denotes Rhodopsin kinase has been difficult to store with retention of activity, but can be successfully stored frozen at -20 degrees C in 20% adonitol.