> top > projects > sentences > docs > PubMed:26581643 > annotations
sentences Home  

PubMed:26581643 JSONTXT 26 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 0-156 Sentence denotes Differential proteomic and genomic profiling of mouse striatal cell model of Huntington's disease and control; probable implications to the disease biology.
T1 0-156 Sentence denotes Differential proteomic and genomic profiling of mouse striatal cell model of Huntington's disease and control; probable implications to the disease biology.
T2 157-168 Sentence denotes UNLABELLED:
T2 157-168 Sentence denotes UNLABELLED:
T3 169-328 Sentence denotes Huntington's disease (HD) is an autosomal dominant disorder of central nervous system caused by expansion of CAG repeats in exon1 of the huntingtin gene (Htt).
T3 169-328 Sentence denotes Huntington's disease (HD) is an autosomal dominant disorder of central nervous system caused by expansion of CAG repeats in exon1 of the huntingtin gene (Htt).
T4 329-493 Sentence denotes Among various dysfunctions originated from the mutation in Htt gene, transcriptional deregulation has been considered to be one of the most important abnormalities.
T4 329-493 Sentence denotes Among various dysfunctions originated from the mutation in Htt gene, transcriptional deregulation has been considered to be one of the most important abnormalities.
T5 494-613 Sentence denotes Large numbers of investigations identified altered expressions of genes in brains of HD patients and many models of HD.
T5 494-613 Sentence denotes Large numbers of investigations identified altered expressions of genes in brains of HD patients and many models of HD.
T6 614-925 Sentence denotes In this study we employed 2D SDS-PAGE/MALDI-MS coupled with 2D-DIGE and real-time PCR experiments of an array of genes focused to HD pathway to determine altered protein and gene expressions in STHdh(Q111)/Hdh(Q111) cells, a cell model of HD and compared with STHdh(Q7)/Hdh(Q7) cells, its wild type counterpart.
T6 614-925 Sentence denotes In this study we employed 2D SDS-PAGE/MALDI-MS coupled with 2D-DIGE and real-time PCR experiments of an array of genes focused to HD pathway to determine altered protein and gene expressions in STHdh(Q111)/Hdh(Q111) cells, a cell model of HD and compared with STHdh(Q7)/Hdh(Q7) cells, its wild type counterpart.
T7 926-1156 Sentence denotes We annotated 76 proteins from these cells and observed differential expressions of 31 proteins (by 2D-DIGE) involved in processes like unfolded protein binding, negative regulation of neuron apoptosis, response to superoxides etc.
T7 926-1156 Sentence denotes We annotated 76 proteins from these cells and observed differential expressions of 31 proteins (by 2D-DIGE) involved in processes like unfolded protein binding, negative regulation of neuron apoptosis, response to superoxides etc.
T8 1157-1226 Sentence denotes Our PCR array experiments identified altered expressions of 47 genes.
T8 1157-1226 Sentence denotes Our PCR array experiments identified altered expressions of 47 genes.
T9 1227-1362 Sentence denotes Altogether significant alteration of 77 genes/proteins could be identified in this HD cell line with potential relevance to HD biology.
T9 1227-1362 Sentence denotes Altogether significant alteration of 77 genes/proteins could be identified in this HD cell line with potential relevance to HD biology.
T10 1363-1387 Sentence denotes BIOLOGICAL SIGNIFICANCE:
T10 1363-1387 Sentence denotes BIOLOGICAL SIGNIFICANCE:
T11 1388-1486 Sentence denotes In this study we intended to find out differential proteomic and genomic profiles in HD condition.
T11 1388-1486 Sentence denotes In this study we intended to find out differential proteomic and genomic profiles in HD condition.
T12 1487-1548 Sentence denotes We used the STHdh cells, a cellular model for HD and control.
T12 1487-1548 Sentence denotes We used the STHdh cells, a cellular model for HD and control.
T13 1549-1656 Sentence denotes These are mouse striatal neuronal cell lines harboring 7 and 111 knock-in CAG repeats in their two alleles.
T13 1549-1656 Sentence denotes These are mouse striatal neuronal cell lines harboring 7 and 111 knock-in CAG repeats in their two alleles.
T14 1657-1829 Sentence denotes The 111Q containing cell line (STHdh(Q111)/Hdh(Q111)) mimics diseased condition, whereas the 7Q containing ones (STHdh(Q7)/Hdh(Q7)), serves as the proper control cell line.
T14 1657-1829 Sentence denotes The 111Q containing cell line (STHdh(Q111)/Hdh(Q111)) mimics diseased condition, whereas the 7Q containing ones (STHdh(Q7)/Hdh(Q7)), serves as the proper control cell line.
T15 1830-2003 Sentence denotes Proteomic experiments were performed earlier to obtain differential expressions of proteins in R6/2 mice models, Hdh(Q) knock-in mice and in plasma and CSF from HD patients.
T15 1830-2003 Sentence denotes Proteomic experiments were performed earlier to obtain differential expressions of proteins in R6/2 mice models, Hdh(Q) knock-in mice and in plasma and CSF from HD patients.
T16 2004-2123 Sentence denotes However, no earlier report on proteomic alterations in these two HD cell lines and control was available in literature.
T16 2004-2123 Sentence denotes However, no earlier report on proteomic alterations in these two HD cell lines and control was available in literature.
T17 2124-2239 Sentence denotes It was, therefore, an important objective to find out differential expressions of proteins in these two cell lines.
T17 2124-2239 Sentence denotes It was, therefore, an important objective to find out differential expressions of proteins in these two cell lines.
T18 2240-2366 Sentence denotes In this study, we annotated 76 proteins from STHdh(Q7)/Hdh(Q7) and STHdh(Q111)/Hdh(Q111) cells using 2D-gel/mass spectrometry.
T18 2240-2366 Sentence denotes In this study, we annotated 76 proteins from STHdh(Q7)/Hdh(Q7) and STHdh(Q111)/Hdh(Q111) cells using 2D-gel/mass spectrometry.
T19 2367-2515 Sentence denotes Next, by performing 2D-DIGE, we observed differential expressions of 31 proteins (16 upregulated and 15 downregulated) between these two cell lines.
T19 2367-2515 Sentence denotes Next, by performing 2D-DIGE, we observed differential expressions of 31 proteins (16 upregulated and 15 downregulated) between these two cell lines.
T20 2516-2701 Sentence denotes We also performed customized qRT-PCR array focused to HD pathway and found differential expressions of 47 genes (8 gene expressions increased and 39 genes were decreased significantly).
T20 2516-2701 Sentence denotes We also performed customized qRT-PCR array focused to HD pathway and found differential expressions of 47 genes (8 gene expressions increased and 39 genes were decreased significantly).
T21 2702-2847 Sentence denotes A total of 77 genes/proteins (Htt downregulated in both the studies) were found to be significantly altered from both the experimental paradigms.
T21 2702-2847 Sentence denotes A total of 77 genes/proteins (Htt downregulated in both the studies) were found to be significantly altered from both the experimental paradigms.
T22 2848-2981 Sentence denotes We validated the differential expressions of Vim, Hypk, Ran, Dstn, Hspa5 and Sod2 either by qRT-PCR or Western blot analysis or both.
T22 2848-2981 Sentence denotes We validated the differential expressions of Vim, Hypk, Ran, Dstn, Hspa5 and Sod2 either by qRT-PCR or Western blot analysis or both.
T23 2982-3109 Sentence denotes Out of these 77, similar trends in alteration of 19 out of 31 and 38 out of 47 proteins/genes were reported in earlier studies.
T23 2982-3109 Sentence denotes Out of these 77, similar trends in alteration of 19 out of 31 and 38 out of 47 proteins/genes were reported in earlier studies.
T24 3110-3225 Sentence denotes Thus our study confirmed earlier observations on differential gene/protein expressions in HD and are really useful.
T24 3110-3225 Sentence denotes Thus our study confirmed earlier observations on differential gene/protein expressions in HD and are really useful.
T25 3226-3305 Sentence denotes Additionally, we observed differential expression of some novel genes/proteins.
T25 3226-3305 Sentence denotes Additionally, we observed differential expression of some novel genes/proteins.
T26 3306-3436 Sentence denotes One of this was Hypk, a Htt-interacting chaperone protein with the ability to solubilize mHtt aggregated structures in cell lines.
T26 3306-3436 Sentence denotes One of this was Hypk, a Htt-interacting chaperone protein with the ability to solubilize mHtt aggregated structures in cell lines.
T27 3437-3545 Sentence denotes We propose that downregulation of Hypk in STHdh(Q111)/Hdh(Q111) has a causal effect towards HD pathogenesis.
T27 3437-3545 Sentence denotes We propose that downregulation of Hypk in STHdh(Q111)/Hdh(Q111) has a causal effect towards HD pathogenesis.
T28 3546-3689 Sentence denotes Thus the novel findings from our study need further research and might be helpful to understand the molecular mechanism behind HD pathogenesis.
T28 3546-3689 Sentence denotes Thus the novel findings from our study need further research and might be helpful to understand the molecular mechanism behind HD pathogenesis.