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PubMed:26269590 JSONTXT 22 Projects

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Id Subject Object Predicate Lexical cue
T1 0-118 Sentence denotes The cAMP Signaling Pathway and Direct Protein Kinase A Phosphorylation Regulate Polycystin-2 (TRPP2) Channel Function.
T2 119-277 Sentence denotes Polycystin-2 (PC2) is a TRP-type, Ca(2+)-permeable non-selective cation channel that plays an important role in Ca(2+) signaling in renal and non-renal cells.
T3 278-432 Sentence denotes The effect(s) of the cAMP pathway and kinase mediated phosphorylation of PC2 seem to be relevant to PC2 trafficking and its interaction with polycystin-1.
T4 433-518 Sentence denotes However, the role of PC2 phosphorylation in channel function is still poorly defined.
T5 519-683 Sentence denotes Here we reconstituted apical membranes of term human syncytiotrophoblast (hST), containing endogenous PC2 (PC2hst), and in vitro translated channel protein (PC2iv).
T6 684-806 Sentence denotes Addition of the catalytic subunit of PKA increased by 566% the spontaneous PC2hst channel activity in the presence of ATP.
T7 807-923 Sentence denotes Interestingly, 8-Br-cAMP also stimulated spontaneous PC2hst channel activity in the absence of the exogenous kinase.
T8 924-1060 Sentence denotes Either stimulation was inhibited by addition of alkaline phosphatase, which in turn, was reversed by the phosphatase inhibitor vanadate.
T9 1061-1163 Sentence denotes Neither maneuver modified the single channel conductance but instead increased channel mean open time.
T10 1164-1286 Sentence denotes PKA directly phosphorylated PC2, which increased the mean open time but not the single channel conductance of the channel.
T11 1287-1384 Sentence denotes PKA phosphorylation did not modify either R742X truncated or S829A-mutant PC2iv channel function.
T12 1385-1476 Sentence denotes The data indicate that the cAMP pathway regulates PC2-mediated cation transport in the hST.
T13 1477-1591 Sentence denotes The relevant PKA site for PC2 channel regulation centers on a single residue serine 829, in the carboxyl terminus.