| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-120 |
Sentence |
denotes |
The nicotine metabolite, cotinine, alters the assembly and trafficking of a subset of nicotinic acetylcholine receptors. |
| T2 |
121-265 |
Sentence |
denotes |
Exposure to nicotine alters the trafficking and assembly of nicotinic receptors (nAChRs), leading to their up-regulation on the plasma membrane. |
| T3 |
266-393 |
Sentence |
denotes |
Although the mechanism is not fully understood, nicotine-induced up-regulation is believed to contribute to nicotine addiction. |
| T4 |
394-526 |
Sentence |
denotes |
The effect of cotinine, the primary metabolite of nicotine, on nAChR trafficking and assembly has not been extensively investigated. |
| T5 |
527-749 |
Sentence |
denotes |
We utilize a pH-sensitive variant of GFP, super ecliptic pHluorin, to differentiate between intracellular nAChRs and those expressed on the plasma membrane to quantify changes resulting from cotinine and nicotine exposure. |
| T6 |
750-909 |
Sentence |
denotes |
Similar to nicotine, exposure to cotinine increases the number of α4β2 receptors on the plasma membrane and causes a redistribution of intracellular receptors. |
| T7 |
910-981 |
Sentence |
denotes |
In contrast to this, cotinine exposure down-regulates α6β2β3 receptors. |
| T8 |
982-1164 |
Sentence |
denotes |
We also used single molecule fluorescence studies to show that cotinine and nicotine both alter the assembly of α4β2 receptors to favor the high sensitivity (α4)2(β2)3 stoichiometry. |
