> top > projects > sentences > docs > PubMed:26265470 > annotations
sentences Home  

PubMed:26265470 JSONTXT 20 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 0-85 Sentence denotes Amino Acid Proximities in Two Sup35 Prion Strains Revealed by Chemical Cross-linking.
T2 86-228 Sentence denotes Strains of the yeast prion [PSI] are different folding patterns of the same Sup35 protein, which stacks up periodically to form a prion fiber.
T3 229-344 Sentence denotes Chemical cross-linking is employed here to probe different fiber structures assembled with a mutant Sup35 fragment.
T4 345-584 Sentence denotes The photo-reactive cross-linker, p-benzoyl-l-phenylalanine (pBpa), was biosynthetically incorporated into bacterially prepared recombinant Sup(1-61)-GFP, containing the first 61 residues of Sup35, followed by the green fluorescent protein.
T5 585-780 Sentence denotes Four methionine substitutions and two alanine substitutions were introduced at fixed positions in Sup(1-61) to allow cyanogen bromide cleavage to facilitate subsequent mass spectrometry analysis.
T6 781-1050 Sentence denotes Amyloid fibers of pBpa and Met/Ala-substituted Sup(1-61)-GFP were nucleated from purified yeast prion particles of two different strains, namely VK and VL, and shown to faithfully transmit specific strain characteristics to yeast expressing the wild type Sup35 protein.
T7 1051-1240 Sentence denotes Intra- and intermolecular cross-linking were distinguished by tandem mass spectrometry analysis on fibers seeded from solutions containing equal amounts of (14)N- and (15)N-labeled protein.
T8 1241-1430 Sentence denotes Fibers propagating the VL strain type exhibited intra- and intermolecular cross-linking between amino acid residues 3 and 28, as well as intra- and intermolecular linking between 32 and 55.
T9 1431-1553 Sentence denotes Inter- and intramolecular cross-linking between residues 32 and 55 were detected in fibers propagating the VK strain type.
T10 1554-1695 Sentence denotes Adjacencies of amino acid residues in space revealed by cross-linking were used to constrain possible chain folds of different [PSI] strains.