| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-146 |
Sentence |
denotes |
The homeodomain transcription factors antennapedia and POU-M2 regulate the transcription of the steroidogenic enzyme gene Phantom in the silkworm. |
| T2 |
147-384 |
Sentence |
denotes |
The steroid hormone ecdysone, which controls insect molting and metamorphosis, is synthesized in the prothoracic gland (PG), and several steroidogenic enzymes that are expressed specifically in the PG are involved in ecdysteroidogenesis. |
| T3 |
385-521 |
Sentence |
denotes |
In this study, we identified new regulators that are involved in the transcriptional control of the silkworm steroidogenic enzyme genes. |
| T4 |
522-729 |
Sentence |
denotes |
In silico analysis predicted several potential cis-regulatory elements (CREs) for the homeodomain transcription factors Antennapedia (Antp) and POU-M2 in the proximal promoters of steroidogenic enzyme genes. |
| T5 |
730-926 |
Sentence |
denotes |
Antp and POU-M2 are expressed dynamically in the PG during larval development, and their overexpression in silkworm embryo-derived (BmE) cells induced the expression of steroidogenic enzyme genes. |
| T6 |
927-1245 |
Sentence |
denotes |
Importantly, luciferase reporter analyses, electrophoretic mobility shift assays, and chromatin immunoprecipitation assays revealed that Antp and POU-M2 promote the transcription of the silkworm steroidogenic enzyme gene Phantom (Phm) by binding directly to specific motifs within overlapping CREs in the Phm promoter. |
| T7 |
1246-1442 |
Sentence |
denotes |
Mutations of these CREs in the Phm promoter suppressed the transcriptional activities of both Antp and POU-M2 in BmE cells and decreased the activities of mutated Phm promoters in the silkworm PG. |
| T8 |
1443-1547 |
Sentence |
denotes |
In addition, pulldown and co-immunoprecipitation assays demonstrated that Antp can interact with POU-M2. |
| T9 |
1548-1734 |
Sentence |
denotes |
Moreover, RNA interference-mediated down-regulation of either Antp or POU-M2 during silkworm wandering not only decreased the ecdysone titer but also led to the failure of metamorphosis. |
| T10 |
1735-1939 |
Sentence |
denotes |
In summary, our results suggest that Antp and POU-M2 coordinate the transcription of the silkworm Phm gene directly, indicating new roles for homeodomain proteins in regulating insect ecdysteroidogenesis. |
