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PubMed:25761366 JSONTXT 86 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-84 Sentence denotes Selective biochemical labeling of Campylobacter jejuni cell-surface glycoconjugates.
T1 0-84 Sentence denotes Selective biochemical labeling of Campylobacter jejuni cell-surface glycoconjugates.
TextSentencer_T2 85-252 Sentence denotes The display of cell-surface glycolipids and glycoproteins is essential for the motility, adhesion and colonization of pathogenic bacteria such as Campylobacter jejuni.
T2 85-252 Sentence denotes The display of cell-surface glycolipids and glycoproteins is essential for the motility, adhesion and colonization of pathogenic bacteria such as Campylobacter jejuni.
TextSentencer_T3 253-516 Sentence denotes Recently, the cell-surface display of C. jejuni glycoconjugates has been the focus of considerable attention; however, our understanding of the roles that glycosylation plays in bacteria still pales in comparison with our understanding of mammalian glycosylation.
T3 253-516 Sentence denotes Recently, the cell-surface display of C. jejuni glycoconjugates has been the focus of considerable attention; however, our understanding of the roles that glycosylation plays in bacteria still pales in comparison with our understanding of mammalian glycosylation.
TextSentencer_T4 517-724 Sentence denotes One of the reasons for this is that carbohydrate metabolic labeling, a powerful tool for studying mammalian glycans, is difficult to establish in bacterial systems and has a significantly more limited scope.
T4 517-724 Sentence denotes One of the reasons for this is that carbohydrate metabolic labeling, a powerful tool for studying mammalian glycans, is difficult to establish in bacterial systems and has a significantly more limited scope.
TextSentencer_T5 725-851 Sentence denotes Herein, we report the development of an alternative strategy that can be used to study bacterial cell-surface glycoconjugates.
T5 725-851 Sentence denotes Herein, we report the development of an alternative strategy that can be used to study bacterial cell-surface glycoconjugates.
TextSentencer_T6 852-965 Sentence denotes Galactose oxidase (GalO) is used to generate an aldehyde at C-6 of terminal GalNAc residues of C. jejuni glycans.
T6 852-965 Sentence denotes Galactose oxidase (GalO) is used to generate an aldehyde at C-6 of terminal GalNAc residues of C. jejuni glycans.
TextSentencer_T7 966-1077 Sentence denotes This newly generated aldehyde can be conjugated with aminooxy-functionalized purification tags or fluorophores.
T7 966-1077 Sentence denotes This newly generated aldehyde can be conjugated with aminooxy-functionalized purification tags or fluorophores.
TextSentencer_T8 1078-1230 Sentence denotes The label can be targeted towards specific glycoconjugates using C. jejuni mutant strains with N-glycan or lipo-oligosaccharides (LOS) assembly defects.
T8 1078-1230 Sentence denotes The label can be targeted towards specific glycoconjugates using C. jejuni mutant strains with N-glycan or lipo-oligosaccharides (LOS) assembly defects.
TextSentencer_T9 1231-1456 Sentence denotes GalO-catalyzed labeling of cell-surface glycoproteins with biotin, allowed for the purification and identification of known extracellular N-linked glycoproteins as well as a recently identified O-linked glycan modifying PorA.
T9 1231-1456 Sentence denotes GalO-catalyzed labeling of cell-surface glycoproteins with biotin, allowed for the purification and identification of known extracellular N-linked glycoproteins as well as a recently identified O-linked glycan modifying PorA.
TextSentencer_T10 1457-1652 Sentence denotes To expand the scope of the GalO reaction, live-cell fluorescent labeling of C. jejuni was used to compare the levels of surface-exposed LOS to the levels of N-glycosylated, cell-surface proteins.
T10 1457-1652 Sentence denotes To expand the scope of the GalO reaction, live-cell fluorescent labeling of C. jejuni was used to compare the levels of surface-exposed LOS to the levels of N-glycosylated, cell-surface proteins.
TextSentencer_T11 1653-1841 Sentence denotes While this study focuses on the GalO-catalyzed labeling of C. jejuni, it can in principle be used to evaluate glycosylation patterns and identify glycoproteins of interest in any bacteria.
T11 1653-1841 Sentence denotes While this study focuses on the GalO-catalyzed labeling of C. jejuni, it can in principle be used to evaluate glycosylation patterns and identify glycoproteins of interest in any bacteria.