> top > projects > sentences > docs > PubMed:2549052 > annotations
sentences Home  

PubMed:2549052 JSONTXT 22 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-61 Sentence denotes Myosin heavy chain kinase from developed Dictyostelium cells.
T1 0-61 Sentence denotes Myosin heavy chain kinase from developed Dictyostelium cells.
T2 62-96 Sentence denotes Purification and characterization.
T2 62-96 Sentence denotes Purification and characterization.
T3 97-272 Sentence denotes We purified to homogeneity the Dictyostelium discoideum myosin heavy chain kinase that is implicated in the heavy chain phosphorylation increases that occur during chemotaxis.
T3 97-272 Sentence denotes We purified to homogeneity the Dictyostelium discoideum myosin heavy chain kinase that is implicated in the heavy chain phosphorylation increases that occur during chemotaxis.
T4 273-348 Sentence denotes The kinase is initially found in the insoluble fraction of developed cells.
T4 273-348 Sentence denotes The kinase is initially found in the insoluble fraction of developed cells.
T5 349-528 Sentence denotes The major purification step was achieved by affinity chromatography using a tail fragment of Dictyostelium myosin (LMM58) expressed in Escherichia coli (De Lozanne, A., Berlot, C.
T5 349-528 Sentence denotes The major purification step was achieved by affinity chromatography using a tail fragment of Dictyostelium myosin (LMM58) expressed in Escherichia coli (De Lozanne, A., Berlot, C.
T6 529-545 Sentence denotes H., Leinwand, L.
T6 529-545 Sentence denotes H., Leinwand, L.
T7 546-565 Sentence denotes A., and Spudich, J.
T7 546-565 Sentence denotes A., and Spudich, J.
T8 566-578 Sentence denotes A. (1988) J.
T8 566-578 Sentence denotes A. (1988) J.
T9 579-589 Sentence denotes Cell Biol.
T9 579-589 Sentence denotes Cell Biol.
T10 590-606 Sentence denotes 105, 2990-3005).
T10 590-606 Sentence denotes 105, 2990-3005).
T11 607-736 Sentence denotes The kinase has an apparent molecular weight of 84,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
T11 607-736 Sentence denotes The kinase has an apparent molecular weight of 84,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
T12 737-803 Sentence denotes The apparent native molecular weight by gel filtration is 240,000.
T12 737-803 Sentence denotes The apparent native molecular weight by gel filtration is 240,000.
T13 804-964 Sentence denotes The kinase catalyzes phosphorylation of myosin heavy chain or LMM58 with similar kinetics, and the extent of phosphorylation for both is 4 mol of phosphate/mol.
T13 804-964 Sentence denotes The kinase catalyzes phosphorylation of myosin heavy chain or LMM58 with similar kinetics, and the extent of phosphorylation for both is 4 mol of phosphate/mol.
T14 965-1044 Sentence denotes With both substrates the Vmax is about 18 mumol/min/mg and the Km is 15 microM.
T14 965-1044 Sentence denotes With both substrates the Vmax is about 18 mumol/min/mg and the Km is 15 microM.
T15 1045-1171 Sentence denotes The myosin heavy chain kinase is specific to Dictyostelium myosin heavy chain, and the phosphorylated amino acid is threonine.
T15 1045-1171 Sentence denotes The myosin heavy chain kinase is specific to Dictyostelium myosin heavy chain, and the phosphorylated amino acid is threonine.
T16 1172-1213 Sentence denotes The kinase undergoes autophosphorylation.
T16 1172-1213 Sentence denotes The kinase undergoes autophosphorylation.
T17 1214-1282 Sentence denotes Each mole of kinase subunit incorporates about 20 mol of phosphates.
T17 1214-1282 Sentence denotes Each mole of kinase subunit incorporates about 20 mol of phosphates.
T18 1283-1443 Sentence denotes Phosphorylation of myosin by this kinase inhibits myosin thick filament formation, suggesting that the kinase plays a role in the regulation of myosin assembly.
T18 1283-1443 Sentence denotes Phosphorylation of myosin by this kinase inhibits myosin thick filament formation, suggesting that the kinase plays a role in the regulation of myosin assembly.