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PubMed:25463755 JSONTXT 44 Projects

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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-144 Sentence denotes SPECHT - single-stage phosphopeptide enrichment and stable-isotope chemical tagging: quantitative phosphoproteomics of insulin action in muscle.
T1 0-144 Sentence denotes SPECHT - single-stage phosphopeptide enrichment and stable-isotope chemical tagging: quantitative phosphoproteomics of insulin action in muscle.
TextSentencer_T2 145-156 Sentence denotes UNLABELLED:
T2 145-156 Sentence denotes UNLABELLED:
TextSentencer_T3 157-261 Sentence denotes The study of cellular signaling remains a significant challenge for translational and clinical research.
T3 157-261 Sentence denotes The study of cellular signaling remains a significant challenge for translational and clinical research.
TextSentencer_T4 262-409 Sentence denotes In particular, robust and accurate methods for quantitative phosphoproteomics in tissues and tumors represent significant hurdles for such efforts.
T4 262-409 Sentence denotes In particular, robust and accurate methods for quantitative phosphoproteomics in tissues and tumors represent significant hurdles for such efforts.
TextSentencer_T5 410-721 Sentence denotes In the present work, we design, implement and validate a method for single-stage phosphopeptide enrichment and stable isotope chemical tagging, or SPECHT, that enables the use of iTRAQ, TMT and/or reductive dimethyl-labeling strategies to be applied to phosphoproteomics experiments performed on primary tissue.
T5 410-721 Sentence denotes In the present work, we design, implement and validate a method for single-stage phosphopeptide enrichment and stable isotope chemical tagging, or SPECHT, that enables the use of iTRAQ, TMT and/or reductive dimethyl-labeling strategies to be applied to phosphoproteomics experiments performed on primary tissue.
TextSentencer_T6 722-952 Sentence denotes We develop and validate our approach using reductive dimethyl-labeling and HeLa cells in culture, and find these results indistinguishable from data generated from more traditional SILAC-labeled HeLa cells mixed at the cell level.
T6 722-952 Sentence denotes We develop and validate our approach using reductive dimethyl-labeling and HeLa cells in culture, and find these results indistinguishable from data generated from more traditional SILAC-labeled HeLa cells mixed at the cell level.
TextSentencer_T7 953-1210 Sentence denotes We apply the SPECHT approach to the quantitative analysis of insulin signaling in a murine myotube cell line and muscle tissue, identify known as well as new phosphorylation events, and validate these phosphorylation sites using phospho-specific antibodies.
T7 953-1210 Sentence denotes We apply the SPECHT approach to the quantitative analysis of insulin signaling in a murine myotube cell line and muscle tissue, identify known as well as new phosphorylation events, and validate these phosphorylation sites using phospho-specific antibodies.
TextSentencer_T8 1211-1372 Sentence denotes Taken together, our work validates chemical tagging post-single-stage phosphoenrichment as a general strategy for studying cellular signaling in primary tissues.
T8 1211-1372 Sentence denotes Taken together, our work validates chemical tagging post-single-stage phosphoenrichment as a general strategy for studying cellular signaling in primary tissues.
TextSentencer_T9 1373-1397 Sentence denotes BIOLOGICAL SIGNIFICANCE:
T9 1373-1397 Sentence denotes BIOLOGICAL SIGNIFICANCE:
TextSentencer_T10 1398-1674 Sentence denotes Through the use of a quantitatively reproducible, proteome-wide phosphopeptide enrichment strategy, we demonstrated the feasibility of post-phosphopeptide purification chemical labeling and tagging as an enabling approach for quantitative phosphoproteomics of primary tissues.
T10 1398-1674 Sentence denotes Through the use of a quantitatively reproducible, proteome-wide phosphopeptide enrichment strategy, we demonstrated the feasibility of post-phosphopeptide purification chemical labeling and tagging as an enabling approach for quantitative phosphoproteomics of primary tissues.
TextSentencer_T11 1675-1928 Sentence denotes Using reductive dimethyl labeling as a generalized chemical tagging strategy, we compared the performance of post-phosphopeptide purification chemical tagging to the well established community standard, SILAC, in insulin-stimulated tissue culture cells.
T11 1675-1928 Sentence denotes Using reductive dimethyl labeling as a generalized chemical tagging strategy, we compared the performance of post-phosphopeptide purification chemical tagging to the well established community standard, SILAC, in insulin-stimulated tissue culture cells.
TextSentencer_T12 1929-2100 Sentence denotes We then extended our method to the analysis of low-dose insulin signaling in murine muscle tissue, and report on the analytical and biological significance of our results.
T12 1929-2100 Sentence denotes We then extended our method to the analysis of low-dose insulin signaling in murine muscle tissue, and report on the analytical and biological significance of our results.