| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-141 |
Sentence |
denotes |
A juvenile hormone transcription factor Bmdimm-fibroin H chain pathway is involved in the synthesis of silk protein in silkworm, Bombyx mori. |
| T2 |
142-264 |
Sentence |
denotes |
The genes responsible for silk biosynthesis are switched on and off at particular times in the silk glands of Bombyx mori. |
| T3 |
265-346 |
Sentence |
denotes |
This switch appears to be under the control of endogenous and exogenous hormones. |
| T4 |
347-475 |
Sentence |
denotes |
However, the molecular mechanisms by which silk protein synthesis is regulated by the juvenile hormone (JH) are largely unknown. |
| T5 |
476-722 |
Sentence |
denotes |
Here, we report a basic helix-loop-helix transcription factor, Bmdimm, its silk gland-specific expression, and its direct involvement in the regulation of fibroin H-chain (fib-H) by binding to an E-box (CAAATG) element of the fib-H gene promoter. |
| T6 |
723-917 |
Sentence |
denotes |
Far-Western blots, enzyme-linked immunosorbent assays, and co-immunoprecipitation assays revealed that Bmdimm protein interacted with another basic helix-loop-helix transcription factor, Bmsage. |
| T7 |
918-1001 |
Sentence |
denotes |
Immunostaining revealed that Bmdimm and Bmsage proteins are co-localized in nuclei. |
| T8 |
1002-1156 |
Sentence |
denotes |
Bmdimm expression was induced in larval silk glands in vivo, in silk glands cultured in vitro, and in B. mori cell lines after treatment with a JH analog. |
| T9 |
1157-1330 |
Sentence |
denotes |
The JH effect on Bmdimm was mediated by the JH-Met-Kr-h1 signaling pathway, and Bmdimm expression did not respond to JH by RNA interference with double-stranded BmKr-h1 RNA. |
| T10 |
1331-1509 |
Sentence |
denotes |
These data suggest that the JH regulatory pathway, the transcription factor Bmdimm, and the targeted fib-H gene contribute to the synthesis of fibroin H-chain protein in B. mori. |
