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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-99 Sentence denotes Rapid identification and quantitative validation of a caffeine-degrading pathway in Pseudomonas sp.
T1 0-99 Sentence denotes Rapid identification and quantitative validation of a caffeine-degrading pathway in Pseudomonas sp.
TextSentencer_T2 100-104 Sentence denotes CES.
T2 100-104 Sentence denotes CES.
TextSentencer_T3 105-272 Sentence denotes Understanding the genes and enzymes involved in caffeine metabolism can lead to applications such as production of methylxanthines and environmental waste remediation.
T3 105-272 Sentence denotes Understanding the genes and enzymes involved in caffeine metabolism can lead to applications such as production of methylxanthines and environmental waste remediation.
TextSentencer_T4 273-288 Sentence denotes Pseudomonas sp.
T4 273-288 Sentence denotes Pseudomonas sp.
TextSentencer_T5 289-523 Sentence denotes CES may provide insights into these applications, since this bacterium degrades caffeine and thrives in concentrations of caffeine that are three times higher (9.0 g L(-1)) than the maximum tolerable levels of other reported bacteria.
T5 289-523 Sentence denotes CES may provide insights into these applications, since this bacterium degrades caffeine and thrives in concentrations of caffeine that are three times higher (9.0 g L(-1)) than the maximum tolerable levels of other reported bacteria.
TextSentencer_T6 524-609 Sentence denotes We took a novel approach toward identifying the enzymatic pathways in Pseudomonas sp.
T6 524-609 Sentence denotes We took a novel approach toward identifying the enzymatic pathways in Pseudomonas sp.
TextSentencer_T7 610-764 Sentence denotes CES that metabolize caffeine, which largely circumvented the need for exhaustive isolation of enzymes and the stepwise reconstitution of their activities.
T7 610-764 Sentence denotes CES that metabolize caffeine, which largely circumvented the need for exhaustive isolation of enzymes and the stepwise reconstitution of their activities.
TextSentencer_T8 765-1151 Sentence denotes Here we describe an optimized, rapid alternative strategy based on multiplexed LC-MS/MS assays and show its application by discovering caffeine-degrading enzymes in the CES strain based on quantitative comparison of proteomes from bacteria grown in the absence and presence of caffeine, the latter condition of which was found to have a highly induced capacity for caffeine degradation.
T8 765-1151 Sentence denotes Here we describe an optimized, rapid alternative strategy based on multiplexed LC-MS/MS assays and show its application by discovering caffeine-degrading enzymes in the CES strain based on quantitative comparison of proteomes from bacteria grown in the absence and presence of caffeine, the latter condition of which was found to have a highly induced capacity for caffeine degradation.
TextSentencer_T9 1152-1434 Sentence denotes Comparisons were made using stable isotope dimethyl labeling, differences in the abundance of particular proteins were substantiated by reciprocal labeling experiments, and the role of the identified proteins in caffeine degradation was independently verified by genetic sequencing.
T9 1152-1434 Sentence denotes Comparisons were made using stable isotope dimethyl labeling, differences in the abundance of particular proteins were substantiated by reciprocal labeling experiments, and the role of the identified proteins in caffeine degradation was independently verified by genetic sequencing.
TextSentencer_T10 1435-1595 Sentence denotes Overall, multiple new components of a N-demethylase system were identified that resulted in rapid pathway validation and gene isolation using this new approach.
T10 1435-1595 Sentence denotes Overall, multiple new components of a N-demethylase system were identified that resulted in rapid pathway validation and gene isolation using this new approach.