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PubMed:24725450 JSONTXT 8 Projects

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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-123 Sentence denotes Conformational changes and translocation of tissue-transglutaminase to the plasma membranes: role in cancer cell migration.
T1 0-123 Sentence denotes Conformational changes and translocation of tissue-transglutaminase to the plasma membranes: role in cancer cell migration.
TextSentencer_T2 124-135 Sentence denotes BACKGROUND:
T2 124-135 Sentence denotes BACKGROUND:
TextSentencer_T3 136-248 Sentence denotes Tissue-transglutaminase (TG2), a dual function G-protein, plays key roles in cell differentiation and migration.
T3 136-248 Sentence denotes Tissue-transglutaminase (TG2), a dual function G-protein, plays key roles in cell differentiation and migration.
TextSentencer_T4 249-335 Sentence denotes In our previous studies we reported the mechanism of TG2-induced cell differentiation.
T4 249-335 Sentence denotes In our previous studies we reported the mechanism of TG2-induced cell differentiation.
TextSentencer_T5 336-425 Sentence denotes In present study, we explored the mechanism of how TG2 may be involved in cell migration.
T5 336-425 Sentence denotes In present study, we explored the mechanism of how TG2 may be involved in cell migration.
TextSentencer_T6 426-434 Sentence denotes METHODS:
T6 426-434 Sentence denotes METHODS:
TextSentencer_T7 435-681 Sentence denotes To study the mechanism of TG2-mediated cell migration, we used neuroblastoma cells (SH-SY5Y) which do not express TG2, neuroblastoma cells expressing exogenous TG2 (SHYTG2), and pancreatic cancer cells which express high levels of endogenous TG2.
T7 435-681 Sentence denotes To study the mechanism of TG2-mediated cell migration, we used neuroblastoma cells (SH-SY5Y) which do not express TG2, neuroblastoma cells expressing exogenous TG2 (SHYTG2), and pancreatic cancer cells which express high levels of endogenous TG2.
TextSentencer_T8 682-869 Sentence denotes Resveratrol, a natural compound previously shown to inhibit neuroblastoma and pancreatic cancer in the animal models, was utilized to investigate the role of TG2 in cancer cell migration.
T8 682-869 Sentence denotes Resveratrol, a natural compound previously shown to inhibit neuroblastoma and pancreatic cancer in the animal models, was utilized to investigate the role of TG2 in cancer cell migration.
TextSentencer_T9 870-1012 Sentence denotes Immunofluorescence assays were employed to detect expression and intracellular localization of TG2, and calcium levels in the migrating cells.
T9 870-1012 Sentence denotes Immunofluorescence assays were employed to detect expression and intracellular localization of TG2, and calcium levels in the migrating cells.
TextSentencer_T10 1013-1159 Sentence denotes Native gel electrophoresis was performed to analyze resveratrol-induced cellular distribution and conformational states of TG2 in migrating cells.
T10 1013-1159 Sentence denotes Native gel electrophoresis was performed to analyze resveratrol-induced cellular distribution and conformational states of TG2 in migrating cells.
TextSentencer_T11 1160-1252 Sentence denotes Data are presented as the mean and standard deviation of at least 3 independent experiments.
T11 1160-1252 Sentence denotes Data are presented as the mean and standard deviation of at least 3 independent experiments.
TextSentencer_T12 1253-1345 Sentence denotes Comparisons were made among groups using one-way ANOVA followed by Tukey-Kramer ad hoc test.
T12 1253-1345 Sentence denotes Comparisons were made among groups using one-way ANOVA followed by Tukey-Kramer ad hoc test.
TextSentencer_T13 1346-1535 Sentence denotes RESULTS: TG2 containing cells (SHYTG2 and pancreatic cancer cells) exhibit increased cell migration and invasion in collagen-coated and matrigel-coated transwell plate assays, respectively.
T13 1346-1535 Sentence denotes RESULTS: TG2 containing cells (SHYTG2 and pancreatic cancer cells) exhibit increased cell migration and invasion in collagen-coated and matrigel-coated transwell plate assays, respectively.
TextSentencer_T14 1536-1605 Sentence denotes Resveratrol (1 μM-10 μM) prevented migration of TG2-expressing cells.
T14 1536-1605 Sentence denotes Resveratrol (1 μM-10 μM) prevented migration of TG2-expressing cells.
TextSentencer_T15 1606-1753 Sentence denotes During the course of migration, resveratrol increased the immunoreactivity of TG2 without affecting the total TG2 protein level in migrating cells.
T15 1606-1753 Sentence denotes During the course of migration, resveratrol increased the immunoreactivity of TG2 without affecting the total TG2 protein level in migrating cells.
TextSentencer_T16 1754-1930 Sentence denotes In these cells, resveratrol increased calcium levels, and depletion of intracellular calcium by a calcium chelator, BAPTA, attenuated resveratrol-enhanced TG2 immunoreactivity.
T16 1754-1930 Sentence denotes In these cells, resveratrol increased calcium levels, and depletion of intracellular calcium by a calcium chelator, BAPTA, attenuated resveratrol-enhanced TG2 immunoreactivity.
TextSentencer_T17 1931-2078 Sentence denotes In native-polyacrylamide gels, we detected an additional TG2 protein band with slower migration in total cell lysates of resveratrol treated cells.
T17 1931-2078 Sentence denotes In native-polyacrylamide gels, we detected an additional TG2 protein band with slower migration in total cell lysates of resveratrol treated cells.
TextSentencer_T18 2079-2282 Sentence denotes This TG2 form is non-phosphorylated, exclusively present in plasma membrane fractions and sensitive to intracellular Ca(2+) concentration suggesting a calcium requirement in TG2-regulated cell migration.
T18 2079-2282 Sentence denotes This TG2 form is non-phosphorylated, exclusively present in plasma membrane fractions and sensitive to intracellular Ca(2+) concentration suggesting a calcium requirement in TG2-regulated cell migration.
TextSentencer_T19 2283-2295 Sentence denotes CONCLUSIONS:
T19 2283-2295 Sentence denotes CONCLUSIONS:
TextSentencer_T20 2296-2521 Sentence denotes Taken together, we conclude that resveratrol induces conformational changes in TG2, and that Ca(2+)-mediated TG2 association with the plasma membrane is responsible for the inhibitory effects of resveratrol on cell migration.
T20 2296-2521 Sentence denotes Taken together, we conclude that resveratrol induces conformational changes in TG2, and that Ca(2+)-mediated TG2 association with the plasma membrane is responsible for the inhibitory effects of resveratrol on cell migration.