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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-77 Sentence denotes RNA sequence analysis of a perinatal lethal osteogenesis imperfecta mutation.
T1 0-77 Sentence denotes RNA sequence analysis of a perinatal lethal osteogenesis imperfecta mutation.
TextSentencer_T2 78-291 Sentence denotes The perinatal lethal form of osteogenesis imperfecta often results from mutations which disrupt stable assembly, delay secretion, and cause excessive posttranslational modification of type I procollagen molecules.
T2 78-291 Sentence denotes The perinatal lethal form of osteogenesis imperfecta often results from mutations which disrupt stable assembly, delay secretion, and cause excessive posttranslational modification of type I procollagen molecules.
TextSentencer_T3 292-387 Sentence denotes One such mutation was efficiently characterized by an indirect method of RNA sequence analysis.
T3 292-387 Sentence denotes One such mutation was efficiently characterized by an indirect method of RNA sequence analysis.
TextSentencer_T4 388-564 Sentence denotes The mutation initially was localized in procollagen by mapping the distribution of abnormal posttranslational modification within the triple helical domain of mutant molecules.
T4 388-564 Sentence denotes The mutation initially was localized in procollagen by mapping the distribution of abnormal posttranslational modification within the triple helical domain of mutant molecules.
TextSentencer_T5 565-850 Sentence denotes Total RNA was isolated from osteogenesis imperfecta cells in culture, cDNA was synthesized using alpha 1(I) and alpha 2(I) specific primers, and fragments of cDNA suspected to harbor the mutation were amplified by the polymerase chain reaction technique and then cloned in M13 vectors.
T5 565-850 Sentence denotes Total RNA was isolated from osteogenesis imperfecta cells in culture, cDNA was synthesized using alpha 1(I) and alpha 2(I) specific primers, and fragments of cDNA suspected to harbor the mutation were amplified by the polymerase chain reaction technique and then cloned in M13 vectors.
TextSentencer_T6 851-1072 Sentence denotes Sequence analysis of the amplified cDNA revealed a new, heterozygous Gly----Val substitution at residue 256 of the triple helical domain of alpha 1(I) chains produced by the perinatal lethal osteogenesis imperfecta cells.
T6 851-1072 Sentence denotes Sequence analysis of the amplified cDNA revealed a new, heterozygous Gly----Val substitution at residue 256 of the triple helical domain of alpha 1(I) chains produced by the perinatal lethal osteogenesis imperfecta cells.
TextSentencer_T7 1073-1174 Sentence denotes The nature and location of the mutation were confirmed by sequence analysis of amplified genomic DNA.
T7 1073-1174 Sentence denotes The nature and location of the mutation were confirmed by sequence analysis of amplified genomic DNA.
TextSentencer_T8 1175-1407 Sentence denotes A Gly----Val substitution has not previously been associated with the lethal form of osteogenesis imperfecta, and this mutation has the most amino-terminal location within the alpha 1(I) chain triple helical domain reported to date.
T8 1175-1407 Sentence denotes A Gly----Val substitution has not previously been associated with the lethal form of osteogenesis imperfecta, and this mutation has the most amino-terminal location within the alpha 1(I) chain triple helical domain reported to date.