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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-181 Sentence denotes Proteomic analysis of interaction between P7-1 of Southern rice black-streaked dwarf virus and the insect vector reveals diverse insect proteins involved in successful transmission.
T1 0-181 Sentence denotes Proteomic analysis of interaction between P7-1 of Southern rice black-streaked dwarf virus and the insect vector reveals diverse insect proteins involved in successful transmission.
TextSentencer_T2 182-193 Sentence denotes UNLABELLED:
T2 182-193 Sentence denotes UNLABELLED:
TextSentencer_T3 194-387 Sentence denotes Southern rice black-streaked dwarf virus (SRBSDV), transmitted by the white-backed planthopper (Sogatella furcifera) in a persistent-propagative manner, has caused serious yield losses in Asia.
T3 194-387 Sentence denotes Southern rice black-streaked dwarf virus (SRBSDV), transmitted by the white-backed planthopper (Sogatella furcifera) in a persistent-propagative manner, has caused serious yield losses in Asia.
TextSentencer_T4 388-547 Sentence denotes Here in a yeast two-hybrid system, protein interactions between SRBSDV P7-1 as a bait protein and a cDNA library of S. furcifera as prey protein were assessed.
T4 388-547 Sentence denotes Here in a yeast two-hybrid system, protein interactions between SRBSDV P7-1 as a bait protein and a cDNA library of S. furcifera as prey protein were assessed.
TextSentencer_T5 548-638 Sentence denotes Of 153 proteins identified as putative interactors, 24 were selected for further analysis.
T5 548-638 Sentence denotes Of 153 proteins identified as putative interactors, 24 were selected for further analysis.
TextSentencer_T6 639-813 Sentence denotes Of the 24 proteins, 18 were further confirmed in a chemiluminescent coimmunoprecipitation (Co-IP) assay as true positive interactors with different strengths of interactions.
T6 639-813 Sentence denotes Of the 24 proteins, 18 were further confirmed in a chemiluminescent coimmunoprecipitation (Co-IP) assay as true positive interactors with different strengths of interactions.
TextSentencer_T7 814-1189 Sentence denotes Six potential candidate proteins (neuroglian, myosin light chain 2 [MLC2], polyubiquitin, E3 ubiquitin ligase, ribophorin ii, and profilin) were analyzed for gene expression in five organs by qRT-PCR; mRNA levels were highest in the gut for neuroglian, MLC2, polyubiquitin and profilin, in the salivary glands for ribophorin ii, and in the haemolymph for E3 ubiquitin ligase.
T7 814-1189 Sentence denotes Six potential candidate proteins (neuroglian, myosin light chain 2 [MLC2], polyubiquitin, E3 ubiquitin ligase, ribophorin ii, and profilin) were analyzed for gene expression in five organs by qRT-PCR; mRNA levels were highest in the gut for neuroglian, MLC2, polyubiquitin and profilin, in the salivary glands for ribophorin ii, and in the haemolymph for E3 ubiquitin ligase.
TextSentencer_T8 1190-1330 Sentence denotes A virus-host protein interaction network was constructed using SRBSDV P7-1 and 18 prey positive protein homologs of Drosophila melanogaster.
T8 1190-1330 Sentence denotes A virus-host protein interaction network was constructed using SRBSDV P7-1 and 18 prey positive protein homologs of Drosophila melanogaster.
TextSentencer_T9 1331-1503 Sentence denotes Our findings suggest that these proteins are involved in the complex host reaction to infection by SRBSDV and provide new insights into the molecular basis of transmission.
T9 1331-1503 Sentence denotes Our findings suggest that these proteins are involved in the complex host reaction to infection by SRBSDV and provide new insights into the molecular basis of transmission.
TextSentencer_T10 1504-1528 Sentence denotes BIOLOGICAL SIGNIFICANCE:
T10 1504-1528 Sentence denotes BIOLOGICAL SIGNIFICANCE:
TextSentencer_T11 1529-1736 Sentence denotes Southern rice black-streaked dwarf virus (SRBSDV), transmitted by S. furcifera in a persistent-propagative manner, is a new found virus and a tentative member of the genus Fijivirus in the family Reoviridae.
T11 1529-1736 Sentence denotes Southern rice black-streaked dwarf virus (SRBSDV), transmitted by S. furcifera in a persistent-propagative manner, is a new found virus and a tentative member of the genus Fijivirus in the family Reoviridae.
TextSentencer_T12 1737-1916 Sentence denotes It was widely noted by plant virologist, government officials and the farmers in Asia in recent years because of its epidemic outbreak and causing serious yield losses after 2009.
T12 1737-1916 Sentence denotes It was widely noted by plant virologist, government officials and the farmers in Asia in recent years because of its epidemic outbreak and causing serious yield losses after 2009.
TextSentencer_T13 1917-2170 Sentence denotes However, the molecular mechanism by which SRBSDV successfully infects and replicates in both plant and insect hosts remains unclear, and much less is known about how the virus spreads from initially infected cells to adjacent cells in the insect vector.
T13 1917-2170 Sentence denotes However, the molecular mechanism by which SRBSDV successfully infects and replicates in both plant and insect hosts remains unclear, and much less is known about how the virus spreads from initially infected cells to adjacent cells in the insect vector.
TextSentencer_T14 2171-2582 Sentence denotes In the present study, we examined protein interactions between SRBSDV P7-1 as the bait and cDNA library of WBPH as the prey by using yeast two-hybrid system, 153 proteins were identified as putative interactors and 24 putative proteins were selected for chemiluminescent coimmunoprecipitation (Co-IP) assay, and then constructed a viral protein-host protein interaction network with homologs of D. melanogaster.
T14 2171-2582 Sentence denotes In the present study, we examined protein interactions between SRBSDV P7-1 as the bait and cDNA library of WBPH as the prey by using yeast two-hybrid system, 153 proteins were identified as putative interactors and 24 putative proteins were selected for chemiluminescent coimmunoprecipitation (Co-IP) assay, and then constructed a viral protein-host protein interaction network with homologs of D. melanogaster.
TextSentencer_T15 2583-2712 Sentence denotes Six WBPH proteins were confirmed as potential P7-1 partners that take part in a pivotal role for viral movement in insect vector.
T15 2583-2712 Sentence denotes Six WBPH proteins were confirmed as potential P7-1 partners that take part in a pivotal role for viral movement in insect vector.
TextSentencer_T16 2713-2832 Sentence denotes These findings will greatly facilitate the understanding of the transmission mechanisms of SRBSDV by its insect vector.
T16 2713-2832 Sentence denotes These findings will greatly facilitate the understanding of the transmission mechanisms of SRBSDV by its insect vector.
TextSentencer_T17 2833-2923 Sentence denotes This is the first to study the molecular interaction between SRBSDV and its insect vector.
T17 2833-2923 Sentence denotes This is the first to study the molecular interaction between SRBSDV and its insect vector.