| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-202 |
Sentence |
denotes |
High glucose-induced O-GlcNAcylated carbohydrate response element-binding protein (ChREBP) mediates mesangial cell lipogenesis and fibrosis: the possible role in the development of diabetic nephropathy. |
| T2 |
203-337 |
Sentence |
denotes |
Carbohydrate response element-binding protein (ChREBP) is a transcription factor responsible for carbohydrate metabolism in the liver. |
| T3 |
338-414 |
Sentence |
denotes |
However, the role of ChREBP in diabetic nephropathy has not been elucidated. |
| T4 |
415-499 |
Sentence |
denotes |
Thus, we investigated the role of ChREBP in mesangial cells in diabetic nephropathy. |
| T5 |
500-657 |
Sentence |
denotes |
Treatment with 25 mM glucose (high glucose; HG) increased cellular O-GlcNAc and O-GlcNAcylated ChREBP in mesangial cells compared with normal 5.5 mM glucose. |
| T6 |
658-921 |
Sentence |
denotes |
O-(2-acetamido-2-deoxy-D-glucopyranosylidene) amino N-phenylcarbamate (PUGNAc), a drug that increases O-GlcNAc, augmented the expression of ChREBP targets, whereas DON, a drug that decreases O-GlcNAc and O-GlcNAcase overexpression, mitigated the increase with HG. |
| T7 |
922-1026 |
Sentence |
denotes |
O-GlcNAc augmented the protein stability, transcriptional activity, and nuclear translocation of ChREBP. |
| T8 |
1027-1143 |
Sentence |
denotes |
HG treatment also stimulated lipid accumulation and the contents of triglyceride and cholesterol in mesangial cells. |
| T9 |
1144-1313 |
Sentence |
denotes |
In addition, HG triggered expression of hypoxia-inducible factor 1-α, vascular endothelial growth factor, and extracellular matrix components related to nephrosclerosis. |
| T10 |
1314-1518 |
Sentence |
denotes |
The ChREBP mutant, W130A, did not exhibit HG-induced lipid accumulation and fibrotic proteins, suggesting that the Trp-130 residue in the MCR3 domain is important in the development of glomerulosclerosis. |
| T11 |
1519-1613 |
Sentence |
denotes |
O-GlcNAcylated ChREBP was elevated in mesangium cells of streptozotocin-induced diabetic rats. |
| T12 |
1614-1770 |
Sentence |
denotes |
In conclusion, HG increased the O-GlcNAcylated ChREBP level, which resulted in lipid accumulation and up-regulation of fibrotic proteins in mesangial cells. |
| T13 |
1771-1846 |
Sentence |
denotes |
These effects may lead mesangial cells to an ultimately pathological state. |
