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PubMed:23999868 JSONTXT 23 Projects

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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-130 Sentence denotes Expression machinery of GM4: the excess amounts of GM3/GM4S synthase (ST3GAL5) are necessary for GM4 synthesis in mammalian cells.
T1 0-130 Sentence denotes Expression machinery of GM4: the excess amounts of GM3/GM4S synthase (ST3GAL5) are necessary for GM4 synthesis in mammalian cells.
TextSentencer_T2 131-250 Sentence denotes The ganglioside GM4 is a sialic acid-containing glycosphingolipid mainly expressed in mammalian brain and erythrocytes.
T2 131-250 Sentence denotes The ganglioside GM4 is a sialic acid-containing glycosphingolipid mainly expressed in mammalian brain and erythrocytes.
TextSentencer_T3 251-410 Sentence denotes GM4 is synthesized by the sialylation of galactosylceramide (GalCer), while the ganglioside GM3 is synthesized by the sialylation of lactosylceramide (LacCer).
T3 251-410 Sentence denotes GM4 is synthesized by the sialylation of galactosylceramide (GalCer), while the ganglioside GM3 is synthesized by the sialylation of lactosylceramide (LacCer).
TextSentencer_T4 411-585 Sentence denotes Recently, the enzyme GM3 synthase was found to be responsible for the synthesis of GM4 in vitro and in vivo, yet the mechanism behind GM4 expression in cells remains unclear.
T4 411-585 Sentence denotes Recently, the enzyme GM3 synthase was found to be responsible for the synthesis of GM4 in vitro and in vivo, yet the mechanism behind GM4 expression in cells remains unclear.
TextSentencer_T5 586-693 Sentence denotes In this study, we attempted to establish GM4-reconstituted cells to reveal the regulation of GM4 synthesis.
T5 586-693 Sentence denotes In this study, we attempted to establish GM4-reconstituted cells to reveal the regulation of GM4 synthesis.
TextSentencer_T6 694-784 Sentence denotes Interestingly, GM4 was not detected in RPMI 1846 cells expressing LacCer, GalCer, and GM3.
T6 694-784 Sentence denotes Interestingly, GM4 was not detected in RPMI 1846 cells expressing LacCer, GalCer, and GM3.
TextSentencer_T7 785-945 Sentence denotes Similarly, GM4 was not detected in CHO-K1 cells, even when such cells expressing LacCer and GM3 were stably transfected with the GalCer synthase (GalCerS) gene.
T7 785-945 Sentence denotes Similarly, GM4 was not detected in CHO-K1 cells, even when such cells expressing LacCer and GM3 were stably transfected with the GalCer synthase (GalCerS) gene.
TextSentencer_T8 946-1086 Sentence denotes GM4 became detectable only when the GM3/GM4 synthase (GM3/GM4S, ST3GAL5) gene was overexpressed in either RPMI 1846 or CHO-K1/GalCerS cells.
T8 946-1086 Sentence denotes GM4 became detectable only when the GM3/GM4 synthase (GM3/GM4S, ST3GAL5) gene was overexpressed in either RPMI 1846 or CHO-K1/GalCerS cells.
TextSentencer_T9 1087-1249 Sentence denotes A mutant of the B16 melanoma cell line, GM-95, lacks GlcCer and LacCer, due to an absence of GlcCer synthase, but carries endogenous LacCer synthase and GM3/GM4S.
T9 1087-1249 Sentence denotes A mutant of the B16 melanoma cell line, GM-95, lacks GlcCer and LacCer, due to an absence of GlcCer synthase, but carries endogenous LacCer synthase and GM3/GM4S.
TextSentencer_T10 1250-1510 Sentence denotes GalCer became detectable after transfection of GalCerS into GM95 cells, but the GM95/GalCerS reconstituted cells did not express GM4, indicating that competition between the substrates LacCer and GalCer for GM3/GM4S does not cause the failure of GM4 synthesis.
T10 1250-1510 Sentence denotes GalCer became detectable after transfection of GalCerS into GM95 cells, but the GM95/GalCerS reconstituted cells did not express GM4, indicating that competition between the substrates LacCer and GalCer for GM3/GM4S does not cause the failure of GM4 synthesis.
TextSentencer_T11 1511-1633 Sentence denotes These results suggest that the expression machinery of GM4 under physiological conditions is independent from that of GM3.
T11 1511-1633 Sentence denotes These results suggest that the expression machinery of GM4 under physiological conditions is independent from that of GM3.