| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-109 |
Sentence |
denotes |
The Lewis X-related α1,3-fucosyltransferase, Fut10, is required for the maintenance of stem cell populations. |
| T2 |
110-331 |
Sentence |
denotes |
Lewis X (Le(X), Galβ1-4(Fucα1-3)GlcNAc) is a carbohydrate epitope that is present at the nonreducing terminus of sugar chains of glycoproteins and glycolipids, and is abundantly expressed in several stem cell populations. |
| T3 |
332-498 |
Sentence |
denotes |
Le(X) antigen can be used in conjunction with fluorescence-activated cell sorting to isolate neurosphere-forming neural stem cells (NSCs) from embryonic mouse brains. |
| T4 |
499-598 |
Sentence |
denotes |
However, its function in the maintenance and differentiation of stem cells remains largely unknown. |
| T5 |
599-758 |
Sentence |
denotes |
In this study, we examined mice deficient for fucosyltransferase 9 (Fut9), which is thought to synthesize most, if not all, of the Le(X) moieties in the brain. |
| T6 |
759-923 |
Sentence |
denotes |
We found that the number of NSCs was increased in the brain of Fut9(-/-) embryos, suggesting that Fut9-synthesized Le(X) is dispensable for the maintenance of NSCs. |
| T7 |
924-1053 |
Sentence |
denotes |
Another α1,3-fucosyltransferase gene, fucosyltransferase 10 (Fut10), is expressed in the ventricular zone of the embryonic brain. |
| T8 |
1054-1112 |
Sentence |
denotes |
Overexpression of Fut10 enhanced the self-renewal of NSCs. |
| T9 |
1113-1218 |
Sentence |
denotes |
Conversely, suppression of Fut10 expression induced the differentiation of NSCs and embryonic stem cells. |
| T10 |
1219-1418 |
Sentence |
denotes |
In addition, knockdown of Fut10 expression in the cortical ventricular zone of the embryonic brain by in utero electroporation of Fut10-miRNAs impaired the radial migration of neural precursor cells. |
| T11 |
1419-1629 |
Sentence |
denotes |
Our data suggest that Fut10 is involved in a unique α1,3-fucosyltransferase activity with stringent substrate specificity, and that this activity is required to maintain stem cells in an undifferentiated state. |
