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PubMed:23339644 JSONTXT 56 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-126 Sentence denotes Characterization of host-cell line specific glycosylation profiles of early transmitted/founder HIV-1 gp120 envelope proteins.
T1 0-126 Sentence denotes Characterization of host-cell line specific glycosylation profiles of early transmitted/founder HIV-1 gp120 envelope proteins.
TextSentencer_T2 127-261 Sentence denotes Glycosylation plays an essential role in regulating protein function by modulating biological, structural, and therapeutic properties.
T2 127-261 Sentence denotes Glycosylation plays an essential role in regulating protein function by modulating biological, structural, and therapeutic properties.
TextSentencer_T3 262-392 Sentence denotes However, due to its inherent heterogeneity and diversity, the comprehensive analysis of protein glycosylation remains a challenge.
T3 262-392 Sentence denotes However, due to its inherent heterogeneity and diversity, the comprehensive analysis of protein glycosylation remains a challenge.
TextSentencer_T4 393-798 Sentence denotes As part of our continuing effort in the analysis of glycosylation profiles of recombinant HIV-1 envelope-based immunogens, we evaluated and compared the host-cell specific glycosylation pattern of recombinant HIV-1 surface glycoprotein, gp120, derived from clade C transmitted/founder virus 1086.C expressed in Chinese hamster ovary (CHO) and human embryonic kidney containing T antigen (293T) cell lines.
T4 393-798 Sentence denotes As part of our continuing effort in the analysis of glycosylation profiles of recombinant HIV-1 envelope-based immunogens, we evaluated and compared the host-cell specific glycosylation pattern of recombinant HIV-1 surface glycoprotein, gp120, derived from clade C transmitted/founder virus 1086.C expressed in Chinese hamster ovary (CHO) and human embryonic kidney containing T antigen (293T) cell lines.
TextSentencer_T5 799-1071 Sentence denotes We used an integrated glycopeptide-based mass mapping workflow that includes a partial deglycosylation step described in our previous study with the inclusion of a fragmentation technique, electron transfer dissociation (ETD), to complement collision-induced dissociation.
T5 799-1071 Sentence denotes We used an integrated glycopeptide-based mass mapping workflow that includes a partial deglycosylation step described in our previous study with the inclusion of a fragmentation technique, electron transfer dissociation (ETD), to complement collision-induced dissociation.
TextSentencer_T6 1072-1274 Sentence denotes The inclusion of ETD facilitated the analysis by providing additional validation for glycopeptide identification and expanding the identified glycopeptides to include coverage of O-linked glycosylation.
T6 1072-1274 Sentence denotes The inclusion of ETD facilitated the analysis by providing additional validation for glycopeptide identification and expanding the identified glycopeptides to include coverage of O-linked glycosylation.
TextSentencer_T7 1275-1438 Sentence denotes The site-specific glycosylation analysis shows that the transmitted/founder 1086.C gp120 expressed in CHO and 293T displayed distinct similarities and differences.
T7 1275-1438 Sentence denotes The site-specific glycosylation analysis shows that the transmitted/founder 1086.C gp120 expressed in CHO and 293T displayed distinct similarities and differences.
TextSentencer_T8 1439-1697 Sentence denotes For N-linked glycosylation, two sites (N386 and N392) in the V4 region were populated with high mannose glycans in the CHO cell-derived 1086.C gp120, while these sites had a mixture of high mannose and processed glycans in the 293T cell-derived 1086.C gp120.
T8 1439-1697 Sentence denotes For N-linked glycosylation, two sites (N386 and N392) in the V4 region were populated with high mannose glycans in the CHO cell-derived 1086.C gp120, while these sites had a mixture of high mannose and processed glycans in the 293T cell-derived 1086.C gp120.
TextSentencer_T9 1698-1923 Sentence denotes Compositional analysis of O-linked glycans revealed that 293T cell-derived 1086.C gp120 consisted of core 1, 2, and 4 type O-linked glycans, while CHO cell-derived 1086.C exclusively consisted of core 1 type O-linked glycans.
T9 1698-1923 Sentence denotes Compositional analysis of O-linked glycans revealed that 293T cell-derived 1086.C gp120 consisted of core 1, 2, and 4 type O-linked glycans, while CHO cell-derived 1086.C exclusively consisted of core 1 type O-linked glycans.
TextSentencer_T10 1924-2087 Sentence denotes Overall, glycosylation site occupancy of the CHO and 293T cell-derived 1086.C gp120 showed a high degree of similarity except for one site at N88 in the C1 region.
T10 1924-2087 Sentence denotes Overall, glycosylation site occupancy of the CHO and 293T cell-derived 1086.C gp120 showed a high degree of similarity except for one site at N88 in the C1 region.
TextSentencer_T11 2088-2167 Sentence denotes This site was partially occupied in 293T-gp120 but fully occupied in CHO-gp120.
T11 2088-2167 Sentence denotes This site was partially occupied in 293T-gp120 but fully occupied in CHO-gp120.
TextSentencer_T12 2168-2382 Sentence denotes Site-specific glycopeptide analysis of transmitted/founder 1086.C gp120 expressed in CHO cells revealed the presence of phosphorylated glycans, while 293T cell-produced 1086.C gp120 glycans were not phosphorylated.
T12 2168-2382 Sentence denotes Site-specific glycopeptide analysis of transmitted/founder 1086.C gp120 expressed in CHO cells revealed the presence of phosphorylated glycans, while 293T cell-produced 1086.C gp120 glycans were not phosphorylated.
TextSentencer_T13 2383-2615 Sentence denotes While the influence of phosphorylated glycans on immunogenicity is unclear, distinguishing host-cell specific variations in glycosylation profiles provide insights into the similarity (or difference) in recombinant vaccine products.
T13 2383-2615 Sentence denotes While the influence of phosphorylated glycans on immunogenicity is unclear, distinguishing host-cell specific variations in glycosylation profiles provide insights into the similarity (or difference) in recombinant vaccine products.
TextSentencer_T14 2616-2837 Sentence denotes While these differences had minimal effect on envelope antigenicity, they may be important in considering immunogenicity and functional capacities of recombinant envelope proteins produced in different expression systems.
T14 2616-2837 Sentence denotes While these differences had minimal effect on envelope antigenicity, they may be important in considering immunogenicity and functional capacities of recombinant envelope proteins produced in different expression systems.