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PubMed:22847002 JSONTXT 37 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-69 Sentence denotes Clostridium perfringens alpha-toxin recognizes the GM1a-TrkA complex.
T2 70-172 Sentence denotes Clostridium perfringens alpha-toxin is the major virulence factor in the pathogenesis of gas gangrene.
T3 173-360 Sentence denotes Alpha-toxin is a 43-kDa protein with two structural domains; the N-domain contains the catalytic site and coordinates the divalent metal ions, and the C-domain is a membrane-binding site.
T4 361-457 Sentence denotes The role of the exposed loop region (72-93 residues) in the N-domain, however, has been unclear.
T5 458-737 Sentence denotes Here we show that this loop contains a ganglioside binding motif (H … SXWY … G) that is the same motif seen in botulinum neurotoxin and directly binds to a specific conformation of the ganglioside Neu5Acα2-3(Galβ1-3GalNAcβ1-4)Galβ1-4Glcβ1Cer (GM1a) through a carbohydrate moiety.
T6 738-906 Sentence denotes Confocal microscopy analysis using fluorescently labeled BODIPY-GM1a revealed that the toxin colocalized with GM1a and induced clustering of GM1a on the cell membranes.
T7 907-1200 Sentence denotes Alpha-toxin was only slightly toxic in β1,4-N-acetylgalactosaminyltransferase knock-out mice, which lack the a-series gangliosides that contain GM1a, but was highly toxic in α2,8-sialyltransferase knock-out mice, which lack both b-series and c-series gangliosides, similar to the control mice.
T8 1201-1401 Sentence denotes Moreover, experiments with site-directed mutants indicated that Trp-84 and Tyr-85 in the exposed alpha-toxin loop play an important role in the interaction with GM1a and subsequent activation of TrkA.
T9 1402-1590 Sentence denotes These results suggest that binding of alpha-toxin to GM1a facilitates the activation of the TrkA receptor and induces a signal transduction cascade that promotes the release of chemokines.
T10 1591-1747 Sentence denotes Therefore, we conclude that GM1a is the primary cellular receptor for alpha-toxin, which can be a potential target for drug developed against this pathogen.