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PubMed:22579746 JSONTXT 35 Projects

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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-88 Sentence denotes Analysis of oxidative stress-induced protein carbonylation using fluorescent hydrazides.
T1 0-88 Sentence denotes Analysis of oxidative stress-induced protein carbonylation using fluorescent hydrazides.
TextSentencer_T2 89-217 Sentence denotes Protein carbonyl detection has been commonly used to analyze the degree of damage to proteins under oxidative stress conditions.
T2 89-217 Sentence denotes Protein carbonyl detection has been commonly used to analyze the degree of damage to proteins under oxidative stress conditions.
TextSentencer_T3 218-390 Sentence denotes Most laboratories rely on derivatization of carbonyl groups with dinitrophenylhydrazine followed by Western blot analysis using antibodies against the dinitrophenyl moiety.
T3 218-390 Sentence denotes Most laboratories rely on derivatization of carbonyl groups with dinitrophenylhydrazine followed by Western blot analysis using antibodies against the dinitrophenyl moiety.
TextSentencer_T4 391-500 Sentence denotes This paper describes a protein carbonyl detection method based on fluorescent Bodipy, Cy3 and Cy5 hydrazides.
T4 391-500 Sentence denotes This paper describes a protein carbonyl detection method based on fluorescent Bodipy, Cy3 and Cy5 hydrazides.
TextSentencer_T5 501-626 Sentence denotes Using this approach, Western blot and immunodetection are no longer needed, shortening the procedure and increasing accuracy.
T5 501-626 Sentence denotes Using this approach, Western blot and immunodetection are no longer needed, shortening the procedure and increasing accuracy.
TextSentencer_T6 627-726 Sentence denotes Combination of Cy3 and Cy5 hydrazides allows multiplexing analyses in a single two-dimensional gel.
T6 627-726 Sentence denotes Combination of Cy3 and Cy5 hydrazides allows multiplexing analyses in a single two-dimensional gel.
TextSentencer_T7 727-956 Sentence denotes Derivatization with Bodipy hydrazide allows easy matching of the spots of interest and those obtained by general fluorescent protein staining methods, which facilitates excising target proteins from the gels and identifying them.
T7 727-956 Sentence denotes Derivatization with Bodipy hydrazide allows easy matching of the spots of interest and those obtained by general fluorescent protein staining methods, which facilitates excising target proteins from the gels and identifying them.
TextSentencer_T8 957-1204 Sentence denotes This method is effective for detecting protein carbonylation in samples of proteins submitted to metal-catalyzed oxidation "in vitro" and assessing the effect of hydrogen peroxide and chronological aging on protein oxidative damage in yeast cells.
T8 957-1204 Sentence denotes This method is effective for detecting protein carbonylation in samples of proteins submitted to metal-catalyzed oxidation "in vitro" and assessing the effect of hydrogen peroxide and chronological aging on protein oxidative damage in yeast cells.