| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-130 |
Sentence |
denotes |
Characterization of arginylation branch of N-end rule pathway in G-protein-mediated proliferation and signaling of cardiomyocytes. |
| T2 |
131-356 |
Sentence |
denotes |
The N-end rule pathway is a proteolytic system in which destabilizing N-terminal amino acids of short lived proteins are recognized by recognition components (N-recognins) as an essential element of degrons, called N-degrons. |
| T3 |
357-569 |
Sentence |
denotes |
In eukaryotes, the major way to generate N-degrons is through arginylation by ATE1 arginyl-tRNA-protein transferases, which transfer Arg from aminoacyl-tRNA to N-terminal Asp and Glu (and Cys as well in mammals). |
| T4 |
570-694 |
Sentence |
denotes |
We have shown previously that ATE1-deficient mice die during embryogenesis with defects in cardiac and vascular development. |
| T5 |
695-782 |
Sentence |
denotes |
Here, we characterized the arginylation-dependent N-end rule pathway in cardiomyocytes. |
| T6 |
783-919 |
Sentence |
denotes |
Our results suggest that the cardiac and vascular defects in ATE1-deficient embryos are independent from each other and cell-autonomous. |
| T7 |
920-1246 |
Sentence |
denotes |
ATE1-deficient myocardium and cardiomyocytes therein, but not non-cardiomyocytes, showed reduced DNA synthesis and mitotic activity ~24 h before the onset of cardiac and vascular defects at embryonic day 12.5 associated with the impairment in the phospholipase C/PKC-MEK1-ERK axis of Gα(q)-mediated cardiac signaling pathways. |
| T8 |
1247-1453 |
Sentence |
denotes |
Cardiac overexpression of Gα(q) rescued ATE1-deficient embryos from thin myocardium and ventricular septal defect but not from vascular defects, genetically dissecting vascular defects from cardiac defects. |
| T9 |
1454-1621 |
Sentence |
denotes |
The misregulation in cardiovascular signaling can be attributed in part to the failure in hypoxia-sensitive degradation of RGS4, a GTPase-activating protein for Gα(q). |
| T10 |
1622-1882 |
Sentence |
denotes |
This study is the first to characterize the N-end rule pathway in cardiomyocytes and reveals the role of its arginylation branch in Gα(q)-mediated signaling of cardiomyocytes in part through N-degron-based, oxygen-sensitive proteolysis of G-protein regulators. |
