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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-140 Sentence denotes Biochemical characterization of WbdN, a β1,3-glucosyltransferase involved in O-antigen synthesis in enterohemorrhagic Escherichia coli O157.
T1 0-140 Sentence denotes Biochemical characterization of WbdN, a β1,3-glucosyltransferase involved in O-antigen synthesis in enterohemorrhagic Escherichia coli O157.
T1 0-140 Sentence denotes Biochemical characterization of WbdN, a β1,3-glucosyltransferase involved in O-antigen synthesis in enterohemorrhagic Escherichia coli O157.
TextSentencer_T2 141-364 Sentence denotes The enterohemorrhagic O157 strain of Escherichia coli, which is one of the most well-known bacterial pathogens, has an O-antigen repeating unit structure with the sequence [-2-d-Rha4NAcα1-3-l-Fucα1-4-d-Glcβ1-3-d-GalNAcα1-].
T2 141-364 Sentence denotes The enterohemorrhagic O157 strain of Escherichia coli, which is one of the most well-known bacterial pathogens, has an O-antigen repeating unit structure with the sequence [-2-d-Rha4NAcα1-3-l-Fucα1-4-d-Glcβ1-3-d-GalNAcα1-].
T2 141-364 Sentence denotes The enterohemorrhagic O157 strain of Escherichia coli, which is one of the most well-known bacterial pathogens, has an O-antigen repeating unit structure with the sequence [-2-d-Rha4NAcα1-3-l-Fucα1-4-d-Glcβ1-3-d-GalNAcα1-].
TextSentencer_T3 365-497 Sentence denotes The O-antigen gene cluster of E. coli O157 contains the genes responsible for the assembly of this repeating unit and includes wbdN.
T3 365-497 Sentence denotes The O-antigen gene cluster of E. coli O157 contains the genes responsible for the assembly of this repeating unit and includes wbdN.
T3 365-497 Sentence denotes The O-antigen gene cluster of E. coli O157 contains the genes responsible for the assembly of this repeating unit and includes wbdN.
TextSentencer_T4 498-635 Sentence denotes In spite of cloning many O-antigen genes, biochemical characterization has been done on very few enzymes involved in O-antigen synthesis.
T4 498-635 Sentence denotes In spite of cloning many O-antigen genes, biochemical characterization has been done on very few enzymes involved in O-antigen synthesis.
T4 498-635 Sentence denotes In spite of cloning many O-antigen genes, biochemical characterization has been done on very few enzymes involved in O-antigen synthesis.
TextSentencer_T5 636-734 Sentence denotes In this work, we expressed the wbdN gene in E. coli BL21, and the His-tagged protein was purified.
T5 636-734 Sentence denotes In this work, we expressed the wbdN gene in E. coli BL21, and the His-tagged protein was purified.
T5 636-734 Sentence denotes In this work, we expressed the wbdN gene in E. coli BL21, and the His-tagged protein was purified.
TextSentencer_T6 735-884 Sentence denotes WbdN activity was characterized using the donor substrate UDP-[(14)C]Glc and the synthetic acceptor substrate GalNAcα-O-PO(3)-PO(3)-(CH(2))(11)-O-Ph.
T6 735-884 Sentence denotes WbdN activity was characterized using the donor substrate UDP-[(14)C]Glc and the synthetic acceptor substrate GalNAcα-O-PO(3)-PO(3)-(CH(2))(11)-O-Ph.
T6 735-1047 Sentence denotes WbdN activity was characterized using the donor substrate UDP-[(14)C]Glc and the synthetic acceptor substrate GalNAcα-O-PO(3)-PO(3)-(CH(2))(11)-O-Ph. The enzyme product was isolated by high pressure liquid chromatography, and mass spectrometry showed that one Glc residue was transferred to the acceptor by WbdN.
TextSentencer_T7 885-1047 Sentence denotes The enzyme product was isolated by high pressure liquid chromatography, and mass spectrometry showed that one Glc residue was transferred to the acceptor by WbdN.
T7 885-1047 Sentence denotes The enzyme product was isolated by high pressure liquid chromatography, and mass spectrometry showed that one Glc residue was transferred to the acceptor by WbdN.
TextSentencer_T8 1048-1154 Sentence denotes Nuclear magnetic resonance analysis of the product structure indicated that Glc was β1-3 linked to GalNAc.
T7 1048-1154 Sentence denotes Nuclear magnetic resonance analysis of the product structure indicated that Glc was β1-3 linked to GalNAc.
T8 1048-1154 Sentence denotes Nuclear magnetic resonance analysis of the product structure indicated that Glc was β1-3 linked to GalNAc.
TextSentencer_T9 1155-1242 Sentence denotes WbdN contains a conserved DxD motif and requires divalent metal ions for full activity.
T8 1155-1242 Sentence denotes WbdN contains a conserved DxD motif and requires divalent metal ions for full activity.
T9 1155-1242 Sentence denotes WbdN contains a conserved DxD motif and requires divalent metal ions for full activity.
TextSentencer_T10 1243-1349 Sentence denotes WbdN activity has an optimal pH between 7 and 8 and is highly specific for UDP-Glc as the donor substrate.
T9 1243-1349 Sentence denotes WbdN activity has an optimal pH between 7 and 8 and is highly specific for UDP-Glc as the donor substrate.
T10 1243-1349 Sentence denotes WbdN activity has an optimal pH between 7 and 8 and is highly specific for UDP-Glc as the donor substrate.
TextSentencer_T11 1350-1507 Sentence denotes GalNAcα derivatives lacking the diphosphate group were inactive as substrates, and the enzyme did not transfer Glc to GlcNAcα-O-PO(3)-PO(3)-(CH(2))(11)-O-Ph.
T11 1350-1507 Sentence denotes GalNAcα derivatives lacking the diphosphate group were inactive as substrates, and the enzyme did not transfer Glc to GlcNAcα-O-PO(3)-PO(3)-(CH(2))(11)-O-Ph.
T10 1350-1610 Sentence denotes GalNAcα derivatives lacking the diphosphate group were inactive as substrates, and the enzyme did not transfer Glc to GlcNAcα-O-PO(3)-PO(3)-(CH(2))(11)-O-Ph. Our results illustrate that WbdN is a specific UDP-Glc:GalNAcα-diphosphate-lipid β1,3-Glc-transferase.
TextSentencer_T12 1508-1610 Sentence denotes Our results illustrate that WbdN is a specific UDP-Glc:GalNAcα-diphosphate-lipid β1,3-Glc-transferase.
T12 1508-1610 Sentence denotes Our results illustrate that WbdN is a specific UDP-Glc:GalNAcα-diphosphate-lipid β1,3-Glc-transferase.
TextSentencer_T13 1611-1700 Sentence denotes The enzyme is a target for the development of inhibitors to block O157-antigen synthesis.
T11 1611-1700 Sentence denotes The enzyme is a target for the development of inhibitors to block O157-antigen synthesis.
T13 1611-1700 Sentence denotes The enzyme is a target for the development of inhibitors to block O157-antigen synthesis.