| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-90 |
Sentence |
denotes |
Expression of biologically active recombinant rat IgE-binding protein in Escherichia coli. |
| T1 |
0-90 |
Sentence |
denotes |
Expression of biologically active recombinant rat IgE-binding protein in Escherichia coli. |
| T2 |
91-233 |
Sentence |
denotes |
IgE-binding protein (epsilon BP) is a protein which has affinity for IgE and was originally identified in rat basophilic leukemia (RBL) cells. |
| T2 |
91-233 |
Sentence |
denotes |
IgE-binding protein (epsilon BP) is a protein which has affinity for IgE and was originally identified in rat basophilic leukemia (RBL) cells. |
| T3 |
234-335 |
Sentence |
denotes |
Subsequently, it was found to be the rat homolog of CBP35, a murine beta-galactoside-specific lectin. |
| T3 |
234-335 |
Sentence |
denotes |
Subsequently, it was found to be the rat homolog of CBP35, a murine beta-galactoside-specific lectin. |
| T4 |
336-436 |
Sentence |
denotes |
This protein is also designated as L-34 and RL-29 and studied independently by several laboratories. |
| T4 |
336-436 |
Sentence |
denotes |
This protein is also designated as L-34 and RL-29 and studied independently by several laboratories. |
| T5 |
437-550 |
Sentence |
denotes |
More recently, CBP35 (epsilon BP) was found to be equivalent to Mac-2, a surface marker on activated macrophages. |
| T5 |
437-550 |
Sentence |
denotes |
More recently, CBP35 (epsilon BP) was found to be equivalent to Mac-2, a surface marker on activated macrophages. |
| T6 |
551-653 |
Sentence |
denotes |
Using rat epsilon BP cDNA, we have succeeded in expressing recombinant epsilon BP in Escherichia coli. |
| T6 |
551-653 |
Sentence |
denotes |
Using rat epsilon BP cDNA, we have succeeded in expressing recombinant epsilon BP in Escherichia coli. |
| T7 |
654-855 |
Sentence |
denotes |
Milligram quantities of homogeneous epsilon BP could be obtained from bacterial lysate in a one-step affinity purification procedure utilizing lactosyl-Sepharose 4B and elution with a lactose gradient. |
| T7 |
654-855 |
Sentence |
denotes |
Milligram quantities of homogeneous epsilon BP could be obtained from bacterial lysate in a one-step affinity purification procedure utilizing lactosyl-Sepharose 4B and elution with a lactose gradient. |
| T8 |
856-1010 |
Sentence |
denotes |
The recombinant epsilon BP (r epsilon BP) binds mouse IgE and retains reactivity to anti-peptide antibodies specific for a sequence within rat epsilon BP. |
| T8 |
856-1010 |
Sentence |
denotes |
The recombinant epsilon BP (r epsilon BP) binds mouse IgE and retains reactivity to anti-peptide antibodies specific for a sequence within rat epsilon BP. |
| T9 |
1011-1315 |
Sentence |
denotes |
The purified r epsilon BP exhibits binding activity to various saccharides, with affinity for N-acetyllactosamine greater than thiodigalactoside greater than lactose much greater than D-galactose greater than L-arabinose, an order identical to that exhibited by native epsilon BP isolated from RBL cells. |
| T9 |
1011-1315 |
Sentence |
denotes |
The purified r epsilon BP exhibits binding activity to various saccharides, with affinity for N-acetyllactosamine greater than thiodigalactoside greater than lactose much greater than D-galactose greater than L-arabinose, an order identical to that exhibited by native epsilon BP isolated from RBL cells. |
| T10 |
1316-1412 |
Sentence |
denotes |
The recombinant lectin displayed hemagglutination activity when tested with rabbit erythrocytes. |
| T10 |
1316-1412 |
Sentence |
denotes |
The recombinant lectin displayed hemagglutination activity when tested with rabbit erythrocytes. |
| T11 |
1413-1654 |
Sentence |
denotes |
Although epsilon BP shares sequence homology to other lectins containing S-type (thiol-dependent) carbohydrate-recognition domains, r epsilon BP is resistant to air oxidation and does not require reducing agents for maintaining its activity. |
| T11 |
1413-1654 |
Sentence |
denotes |
Although epsilon BP shares sequence homology to other lectins containing S-type (thiol-dependent) carbohydrate-recognition domains, r epsilon BP is resistant to air oxidation and does not require reducing agents for maintaining its activity. |
| T12 |
1655-1811 |
Sentence |
denotes |
Furthermore, the single cysteine residue appears to be unexposed and can be alkylated only when the protein is denatured in 5.6 M guanidinium hydrochloride. |
| T12 |
1655-1811 |
Sentence |
denotes |
Furthermore, the single cysteine residue appears to be unexposed and can be alkylated only when the protein is denatured in 5.6 M guanidinium hydrochloride. |
| T13 |
1812-1985 |
Sentence |
denotes |
The availability of a source for a large quantity of epsilon BP should facilitate the analysis of biological function(s) and structure-activity relationships of this lectin. |
| T13 |
1812-1985 |
Sentence |
denotes |
The availability of a source for a large quantity of epsilon BP should facilitate the analysis of biological function(s) and structure-activity relationships of this lectin. |