| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-143 |
Sentence |
denotes |
Purification and identification of naringenin 7-O-methyltransferase, a key enzyme in biosynthesis of flavonoid phytoalexin sakuranetin in rice. |
| T2 |
144-318 |
Sentence |
denotes |
Sakuranetin, the major flavonoid phytoalexin in rice, is induced by ultraviolet (UV) irradiation, CuCl(2) treatment, jasmonic acid treatment, and infection by phytopathogens. |
| T3 |
319-575 |
Sentence |
denotes |
It was recently demonstrated that sakuranetin has anti-inflammatory activity, anti-mutagenic activity, anti-pathogenic activities against Helicobacter pylori, Leishmania, and Trypanosoma and contributes to the maintenance of glucose homeostasis in animals. |
| T4 |
576-674 |
Sentence |
denotes |
Thus, sakuranetin is a useful compound as a plant antibiotic and a potential pharmaceutical agent. |
| T5 |
675-764 |
Sentence |
denotes |
Sakuranetin is biosynthesized from naringenin by naringenin 7-O-methyltransferase (NOMT). |
| T6 |
765-1004 |
Sentence |
denotes |
In previous research, rice NOMT (OsNOMT) was purified to apparent homogeneity from UV-treated wild-type rice leaves, but the purified protein, named OsCOMT1, exhibited caffeic acid O-methyltransferase (COMT) activity and not NOMT activity. |
| T7 |
1005-1153 |
Sentence |
denotes |
In this study, we found that OsCOMT1 does not contribute to sakuranetin production in rice in vivo, and we purified OsNOMT using the oscomt1 mutant. |
| T8 |
1154-1338 |
Sentence |
denotes |
A crude protein preparation from UV-treated oscomt1 leaves was subjected to three sequential purification steps, resulting in a 400-fold purification from the crude enzyme preparation. |
| T9 |
1339-1445 |
Sentence |
denotes |
Using SDS-PAGE, the purest enzyme preparation showed a minor band at an apparent molecular mass of 40 kDa. |
| T10 |
1446-1590 |
Sentence |
denotes |
Two O-methyltransferase-like proteins, encoded by Os04g0175900 and Os12g0240900, were identified from the 40-kDa band by MALDI-TOF/TOF analysis. |
| T11 |
1591-1695 |
Sentence |
denotes |
Recombinant Os12g0240900 protein showed NOMT activity, but the recombinant Os04g0175900 protein did not. |
| T12 |
1696-1885 |
Sentence |
denotes |
Os12g0240900 expression was induced by jasmonic acid treatment in rice leaves prior to sakuranetin accumulation, and the Os12g0240900 protein showed reasonable kinetic properties to OsNOMT. |
| T13 |
1886-1965 |
Sentence |
denotes |
On the basis of these results, we conclude that Os12g0240900 encodes an OsNOMT. |
