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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-147 Sentence denotes New high-performance liquid chromatography assay for glycosyltransferases based on derivatization with anthranilic acid and fluorescence detection.
T1 0-147 Sentence denotes New high-performance liquid chromatography assay for glycosyltransferases based on derivatization with anthranilic acid and fluorescence detection.
T1 0-147 Sentence denotes New high-performance liquid chromatography assay for glycosyltransferases based on derivatization with anthranilic acid and fluorescence detection.
TextSentencer_T2 148-449 Sentence denotes Assays were developed using the unique labeling chemistry of 2-aminobenzoic acid (2AA; anthranilic acid, AA) for measuring activities of both β1-4 galactosyltransferase (GalT-1) and α2-6 sialyltransferase (ST-6) by high-performance liquid chromatography (HPLC) with fluorescence detection (Anumula KR.
T2 148-449 Sentence denotes Assays were developed using the unique labeling chemistry of 2-aminobenzoic acid (2AA; anthranilic acid, AA) for measuring activities of both β1-4 galactosyltransferase (GalT-1) and α2-6 sialyltransferase (ST-6) by high-performance liquid chromatography (HPLC) with fluorescence detection (Anumula KR.
T2 148-449 Sentence denotes Assays were developed using the unique labeling chemistry of 2-aminobenzoic acid (2AA; anthranilic acid, AA) for measuring activities of both β1-4 galactosyltransferase (GalT-1) and α2-6 sialyltransferase (ST-6) by high-performance liquid chromatography (HPLC) with fluorescence detection (Anumula KR.
TextSentencer_T3 450-455 Sentence denotes 2006.
T3 450-455 Sentence denotes 2006.
T3 450-455 Sentence denotes 2006.
TextSentencer_T4 456-587 Sentence denotes Advances in fluorescence derivatization methods for high-performance liquid chromatographic analysis of glycoprotein carbohydrates.
T4 456-587 Sentence denotes Advances in fluorescence derivatization methods for high-performance liquid chromatographic analysis of glycoprotein carbohydrates.
T4 456-587 Sentence denotes Advances in fluorescence derivatization methods for high-performance liquid chromatographic analysis of glycoprotein carbohydrates.
TextSentencer_T5 588-601 Sentence denotes Anal Biochem.
T5 588-601 Sentence denotes Anal Biochem.
T5 588-612 Sentence denotes Anal Biochem. 350:1-23).
TextSentencer_T6 602-612 Sentence denotes 350:1-23).
T6 602-612 Sentence denotes 350:1-23).
TextSentencer_T7 613-837 Sentence denotes N-Acetylglucosamine (GlcNAc) and N-acetyllactosamine were used as acceptors and uridine diphosphate (UDP)-galactose and cytidine monophosphate (CMP)-N-acetylneuraminic acid (NANA) as donors for GalT-1 and ST-6, respectively.
T6 613-837 Sentence denotes N-Acetylglucosamine (GlcNAc) and N-acetyllactosamine were used as acceptors and uridine diphosphate (UDP)-galactose and cytidine monophosphate (CMP)-N-acetylneuraminic acid (NANA) as donors for GalT-1 and ST-6, respectively.
T7 613-837 Sentence denotes N-Acetylglucosamine (GlcNAc) and N-acetyllactosamine were used as acceptors and uridine diphosphate (UDP)-galactose and cytidine monophosphate (CMP)-N-acetylneuraminic acid (NANA) as donors for GalT-1 and ST-6, respectively.
TextSentencer_T8 838-981 Sentence denotes Enzymatic products were labeled in situ with AA and were separated from the substrates on TSKgel Amide 80 column using normal-phase conditions.
T7 838-981 Sentence denotes Enzymatic products were labeled in situ with AA and were separated from the substrates on TSKgel Amide 80 column using normal-phase conditions.
T8 838-981 Sentence denotes Enzymatic products were labeled in situ with AA and were separated from the substrates on TSKgel Amide 80 column using normal-phase conditions.
TextSentencer_T9 982-1134 Sentence denotes Enzyme units were determined from the peak areas by comparison with the concomitantly derivatized standards Gal-β1-4GlcNAc and NANA-α2-6 Gal-β1-4GlcNAc.
T8 982-1134 Sentence denotes Enzyme units were determined from the peak areas by comparison with the concomitantly derivatized standards Gal-β1-4GlcNAc and NANA-α2-6 Gal-β1-4GlcNAc.
T9 982-1134 Sentence denotes Enzyme units were determined from the peak areas by comparison with the concomitantly derivatized standards Gal-β1-4GlcNAc and NANA-α2-6 Gal-β1-4GlcNAc.
TextSentencer_T10 1135-1264 Sentence denotes Linearity (time and enzyme concentration), precision (intra- and interassay) and reproducibility for the assays were established.
T9 1135-1264 Sentence denotes Linearity (time and enzyme concentration), precision (intra- and interassay) and reproducibility for the assays were established.
T10 1135-1264 Sentence denotes Linearity (time and enzyme concentration), precision (intra- and interassay) and reproducibility for the assays were established.
TextSentencer_T11 1265-1370 Sentence denotes The assays were found to be useful in monitoring the enzyme activities during isolation and purification.
T10 1265-1370 Sentence denotes The assays were found to be useful in monitoring the enzyme activities during isolation and purification.
T11 1265-1370 Sentence denotes The assays were found to be useful in monitoring the enzyme activities during isolation and purification.
TextSentencer_T12 1371-1495 Sentence denotes The assays were highly sensitive and performed equal to or better than the traditional radioactive sugar-based measurements.
T11 1371-1495 Sentence denotes The assays were highly sensitive and performed equal to or better than the traditional radioactive sugar-based measurements.
T12 1371-1495 Sentence denotes The assays were highly sensitive and performed equal to or better than the traditional radioactive sugar-based measurements.
TextSentencer_T13 1496-1657 Sentence denotes The assay format can also be used for measuring the activity of other transferases, provided that the carbohydrate acceptors contain a reducing end for labeling.
T12 1496-1657 Sentence denotes The assay format can also be used for measuring the activity of other transferases, provided that the carbohydrate acceptors contain a reducing end for labeling.
T13 1496-1657 Sentence denotes The assay format can also be used for measuring the activity of other transferases, provided that the carbohydrate acceptors contain a reducing end for labeling.
TextSentencer_T14 1658-1718 Sentence denotes An assay for glycoprotein acceptors was developed using IgG.
T13 1658-1718 Sentence denotes An assay for glycoprotein acceptors was developed using IgG.
T14 1658-1718 Sentence denotes An assay for glycoprotein acceptors was developed using IgG.
TextSentencer_T15 1719-1931 Sentence denotes A short HPLC profiling method was developed for the separation of IgG glycans (biantennary G0, G1, G2, mono- and disialylated), which facilitated the determination of GalT-1 and ST-6 activities in a rapid manner.
T14 1719-1931 Sentence denotes A short HPLC profiling method was developed for the separation of IgG glycans (biantennary G0, G1, G2, mono- and disialylated), which facilitated the determination of GalT-1 and ST-6 activities in a rapid manner.
T15 1719-1931 Sentence denotes A short HPLC profiling method was developed for the separation of IgG glycans (biantennary G0, G1, G2, mono- and disialylated), which facilitated the determination of GalT-1 and ST-6 activities in a rapid manner.
TextSentencer_T16 1932-2050 Sentence denotes Furthermore, this profiling method should prove useful for monitoring the changes in IgG glycans in clinical settings.
T15 1932-2050 Sentence denotes Furthermore, this profiling method should prove useful for monitoring the changes in IgG glycans in clinical settings.
T16 1932-2050 Sentence denotes Furthermore, this profiling method should prove useful for monitoring the changes in IgG glycans in clinical settings.