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Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-112 Sentence denotes Rice BGlu1 glycosynthase and wild type transglycosylation activities distinguished by cyclophellitol inhibition.
T1 0-112 Sentence denotes Rice BGlu1 glycosynthase and wild type transglycosylation activities distinguished by cyclophellitol inhibition.
TextSentencer_T2 113-371 Sentence denotes The rice BGlu1 β-D-glucosidase nucleophile mutant E386G is a glycosynthase that catalyzes the synthesis of cellooligosaccharides from α-d-glucopyranosyl fluoride (GlcF) donor and p-nitrophenyl (pNP) cellobioside (Glc2-pNP) or cello-oligosaccharide acceptors.
T2 113-371 Sentence denotes The rice BGlu1 β-D-glucosidase nucleophile mutant E386G is a glycosynthase that catalyzes the synthesis of cellooligosaccharides from α-d-glucopyranosyl fluoride (GlcF) donor and p-nitrophenyl (pNP) cellobioside (Glc2-pNP) or cello-oligosaccharide acceptors.
TextSentencer_T3 372-631 Sentence denotes When activity with other donors and acceptors was tested, the initial enzyme preparation cleaved pNP-β-D-glucopyranoside (Glc-pNP) and pNP-β-D-fucopyranoside (Fuc-pNP) to pNP and glucose and fucose, suggesting contamination with wild type BGlu1 β-glucosidase.
T3 372-631 Sentence denotes When activity with other donors and acceptors was tested, the initial enzyme preparation cleaved pNP-β-D-glucopyranoside (Glc-pNP) and pNP-β-D-fucopyranoside (Fuc-pNP) to pNP and glucose and fucose, suggesting contamination with wild type BGlu1 β-glucosidase.
TextSentencer_T4 632-837 Sentence denotes The products from reaction of GlcF and Fuc-pNP included Fuc-β-(1→3)-Fuc-pNP, Glc-β-(1→3)-Fuc-pNP, and Fuc-β-(1→4)-Glc-β-(1→3)-Fuc-pNP, suggesting the presence of both wild type BGlu1 and its glycosynthase.
T4 632-837 Sentence denotes The products from reaction of GlcF and Fuc-pNP included Fuc-β-(1→3)-Fuc-pNP, Glc-β-(1→3)-Fuc-pNP, and Fuc-β-(1→4)-Glc-β-(1→3)-Fuc-pNP, suggesting the presence of both wild type BGlu1 and its glycosynthase.
TextSentencer_T5 838-1016 Sentence denotes Inhibition of the BGlu1 β-glucosidase activity within this preparation by cyclophellitol confirmed that the E386G glycosynthase preparation was contaminated with wild type BGlu1.
T5 838-1016 Sentence denotes Inhibition of the BGlu1 β-glucosidase activity within this preparation by cyclophellitol confirmed that the E386G glycosynthase preparation was contaminated with wild type BGlu1.
TextSentencer_T6 1017-1194 Sentence denotes Rice BGlu1 E386G-2, generated from a new construct designed to minimize back-mutation, showed glycosynthase activity without wild type hydrolytic or transglycosylation activity.
T6 1017-1194 Sentence denotes Rice BGlu1 E386G-2, generated from a new construct designed to minimize back-mutation, showed glycosynthase activity without wild type hydrolytic or transglycosylation activity.
TextSentencer_T7 1195-1402 Sentence denotes E386G-2 catalyzed transfer of glycosyl residues from GlcF, α-L-arabinosyl fluoride, α-D-fucosyl fluoride, α-D-galactosyl fluoride, α-D-mannosyl fluoride, and α-D-xylosyl fluoride donors to Glc2-pNP acceptor.
T7 1195-1402 Sentence denotes E386G-2 catalyzed transfer of glycosyl residues from GlcF, α-L-arabinosyl fluoride, α-D-fucosyl fluoride, α-D-galactosyl fluoride, α-D-mannosyl fluoride, and α-D-xylosyl fluoride donors to Glc2-pNP acceptor.
TextSentencer_T8 1403-1574 Sentence denotes The synthetic products from the reactions of α-fucosyl fluoride and α-mannosyl fluoride donors were confirmed to result from addition of a β-(1→4)-linked glycosyl residue.
T8 1403-1574 Sentence denotes The synthetic products from the reactions of α-fucosyl fluoride and α-mannosyl fluoride donors were confirmed to result from addition of a β-(1→4)-linked glycosyl residue.
TextSentencer_T9 1575-1790 Sentence denotes Moreover, the E386G glycosynthase transferred glucose from GlcF donor to glucose, cellobiose, Glc-pNP, Fuc-pNP, pNP-β-D-galactopyranoside, and pNP-β-D-xylopyranoside acceptors, but little to pNP-β-D-mannopyranoside.
T9 1575-1790 Sentence denotes Moreover, the E386G glycosynthase transferred glucose from GlcF donor to glucose, cellobiose, Glc-pNP, Fuc-pNP, pNP-β-D-galactopyranoside, and pNP-β-D-xylopyranoside acceptors, but little to pNP-β-D-mannopyranoside.
TextSentencer_T10 1791-1888 Sentence denotes Production of longer oligosaccharides occurred most readily on acceptors with an equatorial 4-OH.
T10 1791-1888 Sentence denotes Production of longer oligosaccharides occurred most readily on acceptors with an equatorial 4-OH.
TextSentencer_T11 1889-2012 Sentence denotes Elimination of wild type contamination thereby allowed a clear assessment of BGlu1 E386G glycosynthase catalytic abilities.
T11 1889-2012 Sentence denotes Elimination of wild type contamination thereby allowed a clear assessment of BGlu1 E386G glycosynthase catalytic abilities.