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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-92 Sentence denotes Analytical constraints for the analysis of human cell line secretomes by shotgun proteomics.
T1 0-92 Sentence denotes Analytical constraints for the analysis of human cell line secretomes by shotgun proteomics.
TextSentencer_T2 93-196 Sentence denotes Human cell line secretome represents a valuable source of therapeutic targets and candidate biomarkers.
T2 93-196 Sentence denotes Human cell line secretome represents a valuable source of therapeutic targets and candidate biomarkers.
TextSentencer_T3 197-289 Sentence denotes Secreted proteins found in biological fluids or culture media are by essence highly diluted.
T3 197-289 Sentence denotes Secreted proteins found in biological fluids or culture media are by essence highly diluted.
TextSentencer_T4 290-432 Sentence denotes Secretome investigation with proteomic approaches is hardly compatible with the high content of proteins found in complete cell culture media.
T4 290-432 Sentence denotes Secretome investigation with proteomic approaches is hardly compatible with the high content of proteins found in complete cell culture media.
TextSentencer_T5 433-516 Sentence denotes Therefore, many studies are currently done with media containing few or no protein.
T5 433-516 Sentence denotes Therefore, many studies are currently done with media containing few or no protein.
TextSentencer_T6 517-579 Sentence denotes Such conditions may perturb cell metabolism and proliferation.
T6 517-579 Sentence denotes Such conditions may perturb cell metabolism and proliferation.
TextSentencer_T7 580-701 Sentence denotes Here, we compared seventeen different compositions of culture media for the human bronchial epithelial BEAS-2B cell line.
T7 580-701 Sentence denotes Here, we compared seventeen different compositions of culture media for the human bronchial epithelial BEAS-2B cell line.
TextSentencer_T8 702-793 Sentence denotes Cell viability, proliferation rate and initial protein charge were systematically compared.
T8 702-793 Sentence denotes Cell viability, proliferation rate and initial protein charge were systematically compared.
TextSentencer_T9 794-963 Sentence denotes We have shown that an important difficulty for the proteomic analysis is due to the presence of detergents such as Pluronic F-68 which hinders peptide mass spectrometry.
T9 794-963 Sentence denotes We have shown that an important difficulty for the proteomic analysis is due to the presence of detergents such as Pluronic F-68 which hinders peptide mass spectrometry.
TextSentencer_T10 964-1096 Sentence denotes The high glucose containing DMEM medium which is free of proteins was shown to preserve a good viability and proliferation of cells.
T10 964-1096 Sentence denotes The high glucose containing DMEM medium which is free of proteins was shown to preserve a good viability and proliferation of cells.
TextSentencer_T11 1097-1202 Sentence denotes With this conditioning medium, we identified 81 extracellular proteins in the secretome of BEAS-2B cells.
T11 1097-1202 Sentence denotes With this conditioning medium, we identified 81 extracellular proteins in the secretome of BEAS-2B cells.
TextSentencer_T12 1203-1355 Sentence denotes Moreover, to illustrate this approach, we exposed BEAS-2B cells to a low toxic dose of CoCl(2,) and found 24 extracellular proteins modulated by cobalt.
T12 1203-1355 Sentence denotes Moreover, to illustrate this approach, we exposed BEAS-2B cells to a low toxic dose of CoCl(2,) and found 24 extracellular proteins modulated by cobalt.
TextSentencer_T13 1356-1458 Sentence denotes This study highlights the possible contribution of such proteomic approach in the field of toxicology.
T13 1356-1458 Sentence denotes This study highlights the possible contribution of such proteomic approach in the field of toxicology.