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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-117 Sentence denotes Production of active human glucocerebrosidase in seeds of Arabidopsis thaliana complex-glycan-deficient (cgl) plants.
T1 0-117 Sentence denotes Production of active human glucocerebrosidase in seeds of Arabidopsis thaliana complex-glycan-deficient (cgl) plants.
T1 0-117 Sentence denotes Production of active human glucocerebrosidase in seeds of Arabidopsis thaliana complex-glycan-deficient (cgl) plants.
TextSentencer_T2 118-249 Sentence denotes There is a clear need for efficient methods to produce protein therapeutics requiring mannose-termination for therapeutic efficacy.
T2 118-249 Sentence denotes There is a clear need for efficient methods to produce protein therapeutics requiring mannose-termination for therapeutic efficacy.
T2 118-249 Sentence denotes There is a clear need for efficient methods to produce protein therapeutics requiring mannose-termination for therapeutic efficacy.
TextSentencer_T3 250-553 Sentence denotes Here we report on a unique system for production of active human lysosomal acid β-glucosidase (glucocerebrosidase, GCase, EC 3.2.1.45) using seeds of the Arabidopsis thaliana complex-glycan-deficient (cgl) mutant, which are deficient in the activity of N-acetylglucosaminyl transferase I (EC 2.4.1.101).
T3 250-553 Sentence denotes Here we report on a unique system for production of active human lysosomal acid β-glucosidase (glucocerebrosidase, GCase, EC 3.2.1.45) using seeds of the Arabidopsis thaliana complex-glycan-deficient (cgl) mutant, which are deficient in the activity of N-acetylglucosaminyl transferase I (EC 2.4.1.101).
T3 250-553 Sentence denotes Here we report on a unique system for production of active human lysosomal acid β-glucosidase (glucocerebrosidase, GCase, EC 3.2.1.45) using seeds of the Arabidopsis thaliana complex-glycan-deficient (cgl) mutant, which are deficient in the activity of N-acetylglucosaminyl transferase I (EC 2.4.1.101).
TextSentencer_T4 554-693 Sentence denotes Gaucher disease is a prevalent lysosomal storage disease in which affected individuals inherit mutations in the gene (GBA1) encoding GCase.
T4 554-693 Sentence denotes Gaucher disease is a prevalent lysosomal storage disease in which affected individuals inherit mutations in the gene (GBA1) encoding GCase.
T4 554-693 Sentence denotes Gaucher disease is a prevalent lysosomal storage disease in which affected individuals inherit mutations in the gene (GBA1) encoding GCase.
TextSentencer_T5 694-876 Sentence denotes A gene cassette optimized for seed expression was used to generate the human enzyme in seeds of the cgl (C5) mutant, and the recombinant GCase was mainly accumulated in the apoplast.
T5 694-876 Sentence denotes A gene cassette optimized for seed expression was used to generate the human enzyme in seeds of the cgl (C5) mutant, and the recombinant GCase was mainly accumulated in the apoplast.
T5 694-876 Sentence denotes A gene cassette optimized for seed expression was used to generate the human enzyme in seeds of the cgl (C5) mutant, and the recombinant GCase was mainly accumulated in the apoplast.
TextSentencer_T6 877-1194 Sentence denotes Importantly, the enzymatic properties including kinetic parameters, half-maximal inhibitory concentration of isofagomine and thermal stability of the cgl-derived GCase were comparable with those of imiglucerase, a commercially available recombinant human GCase used for enzyme replacement therapy in Gaucher patients.
T6 877-1194 Sentence denotes Importantly, the enzymatic properties including kinetic parameters, half-maximal inhibitory concentration of isofagomine and thermal stability of the cgl-derived GCase were comparable with those of imiglucerase, a commercially available recombinant human GCase used for enzyme replacement therapy in Gaucher patients.
T6 877-1194 Sentence denotes Importantly, the enzymatic properties including kinetic parameters, half-maximal inhibitory concentration of isofagomine and thermal stability of the cgl-derived GCase were comparable with those of imiglucerase, a commercially available recombinant human GCase used for enzyme replacement therapy in Gaucher patients.
TextSentencer_T7 1195-1321 Sentence denotes N-glycan structural analyses of recombinant cgl-GCase showed that the majority of the N-glycans (97%) were mannose terminated.
T7 1195-1321 Sentence denotes N-glycan structural analyses of recombinant cgl-GCase showed that the majority of the N-glycans (97%) were mannose terminated.
T7 1195-1321 Sentence denotes N-glycan structural analyses of recombinant cgl-GCase showed that the majority of the N-glycans (97%) were mannose terminated.
TextSentencer_T8 1322-1497 Sentence denotes Additional purification was required to remove ∼15% of the plant-derived recombinant GCase that possessed potentially immunogenic (xylose- and/or fucose-containing) N-glycans.
T8 1322-1497 Sentence denotes Additional purification was required to remove ∼15% of the plant-derived recombinant GCase that possessed potentially immunogenic (xylose- and/or fucose-containing) N-glycans.
T8 1322-1497 Sentence denotes Additional purification was required to remove ∼15% of the plant-derived recombinant GCase that possessed potentially immunogenic (xylose- and/or fucose-containing) N-glycans.
TextSentencer_T9 1498-1583 Sentence denotes Uptake of cgl-derived GCase by mouse macrophages was similar to that of imiglucerase.
T9 1498-1583 Sentence denotes Uptake of cgl-derived GCase by mouse macrophages was similar to that of imiglucerase.
T9 1498-1583 Sentence denotes Uptake of cgl-derived GCase by mouse macrophages was similar to that of imiglucerase.
TextSentencer_T10 1584-1788 Sentence denotes The cgl seed system requires no addition of foreign (non-native) amino acids to the mature recombinant GCase protein, and the dry transgenic seeds represent a stable repository of the therapeutic protein.
T10 1584-1788 Sentence denotes The cgl seed system requires no addition of foreign (non-native) amino acids to the mature recombinant GCase protein, and the dry transgenic seeds represent a stable repository of the therapeutic protein.
T10 1584-1788 Sentence denotes The cgl seed system requires no addition of foreign (non-native) amino acids to the mature recombinant GCase protein, and the dry transgenic seeds represent a stable repository of the therapeutic protein.
TextSentencer_T11 1789-1917 Sentence denotes Other strategies that may completely prevent plant-like complex N-glycans are discussed, including the use of a null cgl mutant.
T11 1789-1917 Sentence denotes Other strategies that may completely prevent plant-like complex N-glycans are discussed, including the use of a null cgl mutant.
T11 1789-1917 Sentence denotes Other strategies that may completely prevent plant-like complex N-glycans are discussed, including the use of a null cgl mutant.