| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-103 |
Sentence |
denotes |
Probing binding pocket of serotonin transporter by single molecular force spectroscopy on living cells. |
| T2 |
104-212 |
Sentence |
denotes |
The serotonin transporter (SERT) terminates neurotransmission by removing serotonin from the synaptic cleft. |
| T3 |
213-352 |
Sentence |
denotes |
In addition, it is the site of action of antidepressants (which block the transporter) and of amphetamines (which induce substrate efflux). |
| T4 |
353-447 |
Sentence |
denotes |
The interaction energies involved in binding of such compounds to the transporter are unknown. |
| T5 |
448-625 |
Sentence |
denotes |
Here, we used atomic force microscopy (AFM) to probe single molecular interactions between the serotonin transporter and MFZ2-12 (a potent cocaine analog) in living CHOK1 cells. |
| T6 |
626-731 |
Sentence |
denotes |
For the AFM measurements, MFZ2-12 was immobilized on AFM tips by using a heterobifunctional cross-linker. |
| T7 |
732-915 |
Sentence |
denotes |
By varying the pulling velocity in force distance cycles drug-transporter complexes were ruptured at different force loadings allowing for mapping of the interaction energy landscape. |
| T8 |
916-1207 |
Sentence |
denotes |
We derived chemical rate constants from these recordings and compared them with those inferred from inhibition of transport and ligand binding: koff values were in good agreement with those derived from uptake experiments; in contrast, the kon values were scaled down when determined by AFM. |
| T9 |
1208-1321 |
Sentence |
denotes |
Our observations generated new insights into the energy landscape of the interaction between SERT and inhibitors. |
| T10 |
1322-1481 |
Sentence |
denotes |
They thus provide a useful framework for molecular dynamics simulations by exploring the range of forces and energies that operate during the binding reaction. |
