| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-145 |
Sentence |
denotes |
Isolation and characterization of extremely minor gangliosides, GM1b and GD1 alpha, in adult bovine brains as developmentally regulated antigens. |
| T1 |
0-145 |
Sentence |
denotes |
Isolation and characterization of extremely minor gangliosides, GM1b and GD1 alpha, in adult bovine brains as developmentally regulated antigens. |
| T2 |
146-346 |
Sentence |
denotes |
In addition to ganglioside GM1b, an unusual and extremely minor ganglioside, GD1 alpha, was efficiently isolated from bovine brain by combination of Q-Sepharose and Iatrobeads column chromatographies. |
| T2 |
146-346 |
Sentence |
denotes |
In addition to ganglioside GM1b, an unusual and extremely minor ganglioside, GD1 alpha, was efficiently isolated from bovine brain by combination of Q-Sepharose and Iatrobeads column chromatographies. |
| T3 |
347-499 |
Sentence |
denotes |
In the course of purification steps, the presence of the sialidase-labile ganglioside was proved by a highly sensitive TLC/enzyme-immunostaining method. |
| T3 |
347-499 |
Sentence |
denotes |
In the course of purification steps, the presence of the sialidase-labile ganglioside was proved by a highly sensitive TLC/enzyme-immunostaining method. |
| T4 |
500-732 |
Sentence |
denotes |
The structure was characterized by gas-liquid chromatography, permethylation study, sialidase degradation, immunostaining with specific antibodies, fast atom bombardment-mass spectrometry, and proton magnetic resonance spectrometry. |
| T4 |
500-732 |
Sentence |
denotes |
The structure was characterized by gas-liquid chromatography, permethylation study, sialidase degradation, immunostaining with specific antibodies, fast atom bombardment-mass spectrometry, and proton magnetic resonance spectrometry. |
| T5 |
733-814 |
Sentence |
denotes |
The content of the ganglioside was very small (0.016%) in the total gangliosides. |
| T5 |
733-814 |
Sentence |
denotes |
The content of the ganglioside was very small (0.016%) in the total gangliosides. |
| T6 |
815-927 |
Sentence |
denotes |
This finding suggests that a synthetic pathway of asialo GM1----GM1b----GD1 alpha may exist in mammalian brains. |
| T6 |
815-927 |
Sentence |
denotes |
This finding suggests that a synthetic pathway of asialo GM1----GM1b----GD1 alpha may exist in mammalian brains. |
| T7 |
928-1148 |
Sentence |
denotes |
A monoclonal antibody NA-6 that was obtained by immunizing mice with purified GM1b reacted specifically with GM1b but showed no cross-reactivity with other structurally related gangliosides such as GM1a, GD1a, and so on. |
| T7 |
928-1148 |
Sentence |
denotes |
A monoclonal antibody NA-6 that was obtained by immunizing mice with purified GM1b reacted specifically with GM1b but showed no cross-reactivity with other structurally related gangliosides such as GM1a, GD1a, and so on. |
| T8 |
1149-1283 |
Sentence |
denotes |
Using the method of TLC/immunostaining with NA-6, GM1b was found to be strongly expressed during embryonic days 14-17 in chick brains. |
| T8 |
1149-1283 |
Sentence |
denotes |
Using the method of TLC/immunostaining with NA-6, GM1b was found to be strongly expressed during embryonic days 14-17 in chick brains. |
| T9 |
1284-1425 |
Sentence |
denotes |
Thus, it is assumed that extremely minor gangliosides like GM1b and GD1 alpha found in adult brains are characterized as embryonic molecules. |
| T9 |
1284-1425 |
Sentence |
denotes |
Thus, it is assumed that extremely minor gangliosides like GM1b and GD1 alpha found in adult brains are characterized as embryonic molecules. |