> top > projects > sentences > docs > PubMed:2168881 > annotations
sentences Home  

PubMed:2168881 JSONTXT 22 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-104 Sentence denotes Substrate specificity of Acanthamoeba myosin I heavy chain kinase as determined with synthetic peptides.
T1 0-104 Sentence denotes Substrate specificity of Acanthamoeba myosin I heavy chain kinase as determined with synthetic peptides.
T2 105-320 Sentence denotes Phosphorylation of a single threonine (myosin IA) or serine (myosins IB and IC) in the heavy chains of the Acanthamoeba myosin I isozymes is required for expression of their actin-activated Mg2(+)-ATPase activities.
T2 105-320 Sentence denotes Phosphorylation of a single threonine (myosin IA) or serine (myosins IB and IC) in the heavy chains of the Acanthamoeba myosin I isozymes is required for expression of their actin-activated Mg2(+)-ATPase activities.
T3 321-525 Sentence denotes We now report that the synthetic peptide Gly-Arg-Gly-Arg-Ser-Ser-Val-Tyr-Ser, which corresponds to the phosphorylated region of Acanthamoeba myosin IC, is a good substrate for myosin I heavy chain kinase:
T3 321-525 Sentence denotes We now report that the synthetic peptide Gly-Arg-Gly-Arg-Ser-Ser-Val-Tyr-Ser, which corresponds to the phosphorylated region of Acanthamoeba myosin IC, is a good substrate for myosin I heavy chain kinase:
T4 526-570 Sentence denotes Km = 54 microM, and Vmax = 15 mumols/min.mg.
T4 526-570 Sentence denotes Km = 54 microM, and Vmax = 15 mumols/min.mg.
T5 571-811 Sentence denotes The same serine is phosphorylated as in the native substrate (residue 6 in the above sequence), and kinase activity with the synthetic peptide as substrate is also stimulated by phosphatidylserine-enhanced autophosphorylation of the kinase.
T5 571-811 Sentence denotes The same serine is phosphorylated as in the native substrate (residue 6 in the above sequence), and kinase activity with the synthetic peptide as substrate is also stimulated by phosphatidylserine-enhanced autophosphorylation of the kinase.
T6 812-949 Sentence denotes These results indicate that all of the essential sequence determinants of kinase specificity are contained within this 9-residue peptide.
T6 812-949 Sentence denotes These results indicate that all of the essential sequence determinants of kinase specificity are contained within this 9-residue peptide.
T7 950-1184 Sentence denotes With the peptide as substrate, we found that another acidic phospholipid, phosphatidylinositol, also enhances autophosphorylation of the kinase whereas the neutral phospholipids phosphatidylcholine and phosphatidylethanolamine do not.
T7 950-1184 Sentence denotes With the peptide as substrate, we found that another acidic phospholipid, phosphatidylinositol, also enhances autophosphorylation of the kinase whereas the neutral phospholipids phosphatidylcholine and phosphatidylethanolamine do not.
T8 1185-1472 Sentence denotes By comparing the Km and Vmax values for a series of synthetic peptide substrates, we established that 1 basic amino acid is essential on the NH2-terminal side of the phosphorylation site, and two are preferable, and that a tyrosine is essential 2 residues away on the COOH-terminal side.
T8 1185-1472 Sentence denotes By comparing the Km and Vmax values for a series of synthetic peptide substrates, we established that 1 basic amino acid is essential on the NH2-terminal side of the phosphorylation site, and two are preferable, and that a tyrosine is essential 2 residues away on the COOH-terminal side.
T9 1473-1529 Sentence denotes There is a slight preference for arginines over lysines.
T9 1473-1529 Sentence denotes There is a slight preference for arginines over lysines.
T10 1530-1751 Sentence denotes All of these local sequence specificity determinants are present in the three native substrates, Acanthamoeba myosins IA, IB, and IC, and in two Dictyostelium myosin I isozymes that are putative substrates for the kinase.
T10 1530-1751 Sentence denotes All of these local sequence specificity determinants are present in the three native substrates, Acanthamoeba myosins IA, IB, and IC, and in two Dictyostelium myosin I isozymes that are putative substrates for the kinase.
T11 1752-1883 Sentence denotes Similar sequences do not occur in the myosins I from intestinal brush border, which is not a substrate for the Acanthamoeba kinase.
T11 1752-1883 Sentence denotes Similar sequences do not occur in the myosins I from intestinal brush border, which is not a substrate for the Acanthamoeba kinase.