| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-71 |
Sentence |
denotes |
Binding of Clostridium difficile toxins to human milk oligosaccharides. |
| T1 |
0-71 |
Sentence |
denotes |
Binding of Clostridium difficile toxins to human milk oligosaccharides. |
| T1 |
0-71 |
Sentence |
denotes |
Binding of Clostridium difficile toxins to human milk oligosaccharides. |
| TextSentencer_T2 |
72-464 |
Sentence |
denotes |
The binding of recombinant fragments of the C-terminal cell-binding domains of the two large exotoxins, toxin A (TcdA) and toxin B (TcdB), expressed by Clostridium difficile and a library consisting of the most abundant neutral and acidic human milk oligosaccharides (HMOs) was examined quantitatively at 25°C and pH 7 using the direct electrospray ionization mass spectrometry (ES-MS) assay. |
| T2 |
72-464 |
Sentence |
denotes |
The binding of recombinant fragments of the C-terminal cell-binding domains of the two large exotoxins, toxin A (TcdA) and toxin B (TcdB), expressed by Clostridium difficile and a library consisting of the most abundant neutral and acidic human milk oligosaccharides (HMOs) was examined quantitatively at 25°C and pH 7 using the direct electrospray ionization mass spectrometry (ES-MS) assay. |
| T2 |
72-464 |
Sentence |
denotes |
The binding of recombinant fragments of the C-terminal cell-binding domains of the two large exotoxins, toxin A (TcdA) and toxin B (TcdB), expressed by Clostridium difficile and a library consisting of the most abundant neutral and acidic human milk oligosaccharides (HMOs) was examined quantitatively at 25°C and pH 7 using the direct electrospray ionization mass spectrometry (ES-MS) assay. |
| TextSentencer_T3 |
465-713 |
Sentence |
denotes |
The results of the ES-MS measurements indicate that both toxin fragments investigated, TcdB-B1 and TcdA-A2, which possess one and two carbohydrate binding sites, respectively, bind specifically to HMOs ranging in size from tri- to heptasaccharides. |
| T3 |
465-713 |
Sentence |
denotes |
The results of the ES-MS measurements indicate that both toxin fragments investigated, TcdB-B1 and TcdA-A2, which possess one and two carbohydrate binding sites, respectively, bind specifically to HMOs ranging in size from tri- to heptasaccharides. |
| T3 |
465-713 |
Sentence |
denotes |
The results of the ES-MS measurements indicate that both toxin fragments investigated, TcdB-B1 and TcdA-A2, which possess one and two carbohydrate binding sites, respectively, bind specifically to HMOs ranging in size from tri- to heptasaccharides. |
| TextSentencer_T4 |
714-767 |
Sentence |
denotes |
Notably, five of the HMOs tested bind to both toxins: |
| T4 |
714-767 |
Sentence |
denotes |
Notably, five of the HMOs tested bind to both toxins: |
| T4 |
714-767 |
Sentence |
denotes |
Notably, five of the HMOs tested bind to both toxins: |
| TextSentencer_T5 |
768-964 |
Sentence |
denotes |
Fuc(α1-2)Gal(β1-4)Glc, Gal(β1-3)GlcNAc(β1-3)Gal(β1-4)Glc, Fuc(α1-2)Gal(β1-3)GlcNAc(β1-3)Gal(β1-4)Glc, Gal(β1-3)[Fuc(α1-4)]GlcNAc(β1-3)Gal(β1-4)Glc and Gal(β1-4)[Fuc(α1-3)]GlcNAc(β1-3)Gal(β1-4)Glc. |
| T5 |
768-964 |
Sentence |
denotes |
Fuc(α1-2)Gal(β1-4)Glc, Gal(β1-3)GlcNAc(β1-3)Gal(β1-4)Glc, Fuc(α1-2)Gal(β1-3)GlcNAc(β1-3)Gal(β1-4)Glc, Gal(β1-3)[Fuc(α1-4)]GlcNAc(β1-3)Gal(β1-4)Glc and Gal(β1-4)[Fuc(α1-3)]GlcNAc(β1-3)Gal(β1-4)Glc. |
| T5 |
768-1055 |
Sentence |
denotes |
Fuc(α1-2)Gal(β1-4)Glc, Gal(β1-3)GlcNAc(β1-3)Gal(β1-4)Glc, Fuc(α1-2)Gal(β1-3)GlcNAc(β1-3)Gal(β1-4)Glc, Gal(β1-3)[Fuc(α1-4)]GlcNAc(β1-3)Gal(β1-4)Glc and Gal(β1-4)[Fuc(α1-3)]GlcNAc(β1-3)Gal(β1-4)Glc. However, the binding of the HMOs is uniformly weak, with apparent affinities ≤10(3 )M(-1). |
| TextSentencer_T6 |
965-1055 |
Sentence |
denotes |
However, the binding of the HMOs is uniformly weak, with apparent affinities ≤10(3 )M(-1). |
| T6 |
965-1055 |
Sentence |
denotes |
However, the binding of the HMOs is uniformly weak, with apparent affinities ≤10(3 )M(-1). |
| TextSentencer_T7 |
1056-1282 |
Sentence |
denotes |
The results of molecular docking simulations, taken together with the experimental binding data, suggest that a disaccharide moiety (lactose or lactosamine) represents the core HMO recognition element for both toxin fragments. |
| T6 |
1056-1282 |
Sentence |
denotes |
The results of molecular docking simulations, taken together with the experimental binding data, suggest that a disaccharide moiety (lactose or lactosamine) represents the core HMO recognition element for both toxin fragments. |
| T7 |
1056-1282 |
Sentence |
denotes |
The results of molecular docking simulations, taken together with the experimental binding data, suggest that a disaccharide moiety (lactose or lactosamine) represents the core HMO recognition element for both toxin fragments. |
| TextSentencer_T8 |
1283-1422 |
Sentence |
denotes |
The results of a Verocytotoxicity neutralization assay reveal that HMOs do not significantly inhibit the cytotoxic effects of TcdA or TcdB. |
| T7 |
1283-1422 |
Sentence |
denotes |
The results of a Verocytotoxicity neutralization assay reveal that HMOs do not significantly inhibit the cytotoxic effects of TcdA or TcdB. |
| T8 |
1283-1422 |
Sentence |
denotes |
The results of a Verocytotoxicity neutralization assay reveal that HMOs do not significantly inhibit the cytotoxic effects of TcdA or TcdB. |
| TextSentencer_T9 |
1423-1542 |
Sentence |
denotes |
The absence of protection is attributed to the very weak intrinsic affinities that the toxins exhibit towards the HMOs. |
| T8 |
1423-1542 |
Sentence |
denotes |
The absence of protection is attributed to the very weak intrinsic affinities that the toxins exhibit towards the HMOs. |
| T9 |
1423-1542 |
Sentence |
denotes |
The absence of protection is attributed to the very weak intrinsic affinities that the toxins exhibit towards the HMOs. |
