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PubMed:2159009 JSONTXT 36 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-46 Sentence denotes Generation of cell surface neoganglioproteins.
T1 0-46 Sentence denotes Generation of cell surface neoganglioproteins.
T2 47-117 Sentence denotes GM1-neoganglioproteins are non-functional receptors for cholera toxin.
T2 47-117 Sentence denotes GM1-neoganglioproteins are non-functional receptors for cholera toxin.
T3 118-369 Sentence denotes GM1 (II3Neu5Ac-GgOse4Cer)-oligosaccharide was prepared from the ganglioside by ozonolysis and alkaline fragmentation, reductively aminated and coupled to the heterobifunctional cross-linker succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate.
T3 118-369 Sentence denotes GM1 (II3Neu5Ac-GgOse4Cer)-oligosaccharide was prepared from the ganglioside by ozonolysis and alkaline fragmentation, reductively aminated and coupled to the heterobifunctional cross-linker succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate.
T4 370-530 Sentence denotes The resulting derivative reacted with free sulfhydryl groups and readily cross-linked to cell surface components on rat glioma C6 cells which are GM1-deficient.
T4 370-530 Sentence denotes The resulting derivative reacted with free sulfhydryl groups and readily cross-linked to cell surface components on rat glioma C6 cells which are GM1-deficient.
T5 531-701 Sentence denotes Attachment of the GM1-oligosaccharide derivative, which was monitored by increased binding of 125I-cholera toxin to the cells, was both time- and concentration-dependent.
T5 531-701 Sentence denotes Attachment of the GM1-oligosaccharide derivative, which was monitored by increased binding of 125I-cholera toxin to the cells, was both time- and concentration-dependent.
T6 702-823 Sentence denotes Prior treatment of the cells with dithiothreitol enhanced the attachment by generating additional free sulfhydryl groups.
T6 702-823 Sentence denotes Prior treatment of the cells with dithiothreitol enhanced the attachment by generating additional free sulfhydryl groups.
T7 824-936 Sentence denotes The affinity of cholera toxin for cells treated with the GM1-oligosaccharide derivative or with GM1 was similar.
T7 824-936 Sentence denotes The affinity of cholera toxin for cells treated with the GM1-oligosaccharide derivative or with GM1 was similar.
T8 937-1022 Sentence denotes The nature of the newly generated toxin receptors was determined by Western blotting.
T8 937-1022 Sentence denotes The nature of the newly generated toxin receptors was determined by Western blotting.
T9 1023-1267 Sentence denotes Membranes from derivatized cells were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the resolved components were electrophoretically transferred to a nitrocellulose sheet which was overlain with 125I-cholera toxin.
T9 1023-1267 Sentence denotes Membranes from derivatized cells were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the resolved components were electrophoretically transferred to a nitrocellulose sheet which was overlain with 125I-cholera toxin.
T10 1268-1361 Sentence denotes The toxin bound to a wide variety of membrane proteins, most of which were trypsin-sensitive.
T10 1268-1361 Sentence denotes The toxin bound to a wide variety of membrane proteins, most of which were trypsin-sensitive.
T11 1362-1426 Sentence denotes No such binding was observed using membranes from control cells.
T11 1362-1426 Sentence denotes No such binding was observed using membranes from control cells.
T12 1427-1685 Sentence denotes Although the GM1-neoganglioproteins newly generated on the surface of rat glioma C6 cells readily bound cholera toxin, the cells did not become more responsive to the toxin as measured by increased production of cyclic AMP or activation of adenylate cyclase.
T12 1427-1685 Sentence denotes Although the GM1-neoganglioproteins newly generated on the surface of rat glioma C6 cells readily bound cholera toxin, the cells did not become more responsive to the toxin as measured by increased production of cyclic AMP or activation of adenylate cyclase.
T13 1686-1758 Sentence denotes In contrast, cells exposed to GM1 became highly responsive to the toxin.
T13 1686-1758 Sentence denotes In contrast, cells exposed to GM1 became highly responsive to the toxin.
T14 1759-1866 Sentence denotes Thus, neoganglioproteins on the cell surface appear to behave as nonfunctional receptors for cholera toxin.
T14 1759-1866 Sentence denotes Thus, neoganglioproteins on the cell surface appear to behave as nonfunctional receptors for cholera toxin.