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PubMed:2136386 / 474-1231 JSONTXT 14 Projects

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Id Subject Object Predicate Lexical cue
TextSentencer_T5 0-106 Sentence denotes The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia.
T5 0-106 Sentence denotes The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia.
T5 0-757 Sentence denotes The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia. The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia. Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia. These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C. In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C. However, no Sia was detected after growth at 15 degrees C. A temperature upshift experiment showed that the intracellular concentration of Sia increased ca.
TextSentencer_T6 107-236 Sentence denotes The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia.
T6 107-236 Sentence denotes The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia.
TextSentencer_T7 237-331 Sentence denotes Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia.
T7 237-331 Sentence denotes Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia.
TextSentencer_T8 332-464 Sentence denotes These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C.
T8 332-464 Sentence denotes These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C.
TextSentencer_T9 465-600 Sentence denotes In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C.
T9 465-600 Sentence denotes In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C.
TextSentencer_T10 601-659 Sentence denotes However, no Sia was detected after growth at 15 degrees C.
T10 601-659 Sentence denotes However, no Sia was detected after growth at 15 degrees C.
TextSentencer_T11 660-757 Sentence denotes A temperature upshift experiment showed that the intracellular concentration of Sia increased ca.
T11 660-757 Sentence denotes A temperature upshift experiment showed that the intracellular concentration of Sia increased ca.