> top > projects > sentences > docs > PubMed:2136386 > annotations

PubMed:2136386 JSONTXT 51 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-67 Sentence denotes Biosynthesis of the polysialic acid capsule in Escherichia coli K1.
T1 0-67 Sentence denotes Biosynthesis of the polysialic acid capsule in Escherichia coli K1.
T1 0-67 Sentence denotes Biosynthesis of the polysialic acid capsule in Escherichia coli K1.
TextSentencer_T2 68-158 Sentence denotes Cold inactivation of sialic acid synthase regulates capsule expression below 20 degrees C.
T2 68-158 Sentence denotes Cold inactivation of sialic acid synthase regulates capsule expression below 20 degrees C.
T2 68-158 Sentence denotes Cold inactivation of sialic acid synthase regulates capsule expression below 20 degrees C.
TextSentencer_T3 159-329 Sentence denotes When neuroinvasive Escherichia coli K1 cells are grown at temperatures below 20 degrees C, they fail to synthesize the alpha-2,8-linked polysialic acid (polySia) capsule.
T3 159-329 Sentence denotes When neuroinvasive Escherichia coli K1 cells are grown at temperatures below 20 degrees C, they fail to synthesize the alpha-2,8-linked polysialic acid (polySia) capsule.
T3 159-329 Sentence denotes When neuroinvasive Escherichia coli K1 cells are grown at temperatures below 20 degrees C, they fail to synthesize the alpha-2,8-linked polysialic acid (polySia) capsule.
TextSentencer_T4 330-473 Sentence denotes The objective of this study was to use a genetic and biochemical approach to analyse why capsule expression was defective at cold temperatures.
T4 330-473 Sentence denotes The objective of this study was to use a genetic and biochemical approach to analyse why capsule expression was defective at cold temperatures.
T4 330-473 Sentence denotes The objective of this study was to use a genetic and biochemical approach to analyse why capsule expression was defective at cold temperatures.
TextSentencer_T5 474-580 Sentence denotes The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia.
T5 474-580 Sentence denotes The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia.
T5 474-1231 Sentence denotes The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia. The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia. Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia. These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C. In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C. However, no Sia was detected after growth at 15 degrees C. A temperature upshift experiment showed that the intracellular concentration of Sia increased ca.
TextSentencer_T6 581-710 Sentence denotes The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia.
T6 581-710 Sentence denotes The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia.
TextSentencer_T7 711-805 Sentence denotes Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia.
T7 711-805 Sentence denotes Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia.
TextSentencer_T8 806-938 Sentence denotes These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C.
T8 806-938 Sentence denotes These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C.
TextSentencer_T9 939-1074 Sentence denotes In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C.
T9 939-1074 Sentence denotes In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C.
TextSentencer_T10 1075-1133 Sentence denotes However, no Sia was detected after growth at 15 degrees C.
T10 1075-1133 Sentence denotes However, no Sia was detected after growth at 15 degrees C.
TextSentencer_T11 1134-1231 Sentence denotes A temperature upshift experiment showed that the intracellular concentration of Sia increased ca.
T11 1134-1231 Sentence denotes A temperature upshift experiment showed that the intracellular concentration of Sia increased ca.
TextSentencer_T12 1232-1376 Sentence denotes 3-fold within 5-10 min after shift from 15 to 37 degrees C, even in the presence of inhibitors of protein synthesis or transcription initiation.
T6 1232-1376 Sentence denotes 3-fold within 5-10 min after shift from 15 to 37 degrees C, even in the presence of inhibitors of protein synthesis or transcription initiation.
T12 1232-1376 Sentence denotes 3-fold within 5-10 min after shift from 15 to 37 degrees C, even in the presence of inhibitors of protein synthesis or transcription initiation.
TextSentencer_T13 1377-1641 Sentence denotes An in vitro assay for Sia synthase showed that Sia was synthesized at 37 degrees C in cell-free extracts from both 37 and 15 degrees C grown cells, but that no synthesis occurred when the same extracts were assayed at 15 degrees C.(ABSTRACT TRUNCATED AT 250 WORDS)
T7 1377-1641 Sentence denotes An in vitro assay for Sia synthase showed that Sia was synthesized at 37 degrees C in cell-free extracts from both 37 and 15 degrees C grown cells, but that no synthesis occurred when the same extracts were assayed at 15 degrees C.(ABSTRACT TRUNCATED AT 250 WORDS)
T13 1377-1641 Sentence denotes An in vitro assay for Sia synthase showed that Sia was synthesized at 37 degrees C in cell-free extracts from both 37 and 15 degrees C grown cells, but that no synthesis occurred when the same extracts were assayed at 15 degrees C.(ABSTRACT TRUNCATED AT 250 WORDS)