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PubMed:2115517 JSONTXT 37 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-101 Sentence denotes Synthesis of chondroitin sulfate D and heparin proteoglycans in murine lymph node-derived mast cells.
T1 0-101 Sentence denotes Synthesis of chondroitin sulfate D and heparin proteoglycans in murine lymph node-derived mast cells.
T2 102-132 Sentence denotes The dependence on fibroblasts.
T2 102-132 Sentence denotes The dependence on fibroblasts.
T3 133-248 Sentence denotes Proteoglycans synthesized in cultured mast cells derived from horse serum-immunized lymph node cells were analyzed.
T3 133-248 Sentence denotes Proteoglycans synthesized in cultured mast cells derived from horse serum-immunized lymph node cells were analyzed.
T4 249-515 Sentence denotes Treatment of the 35S-proteoglycans extracted from these cells with either chondroitinase ABC or AC resulted in 95% +/- 7% and 84% +/- 7%, respectively (mean +/- S.E., n = 3), of the radioactivity associated with disaccharides eluting in the included volume of PD-10.
T4 249-515 Sentence denotes Treatment of the 35S-proteoglycans extracted from these cells with either chondroitinase ABC or AC resulted in 95% +/- 7% and 84% +/- 7%, respectively (mean +/- S.E., n = 3), of the radioactivity associated with disaccharides eluting in the included volume of PD-10.
T5 516-596 Sentence denotes The 35S-proteoglycans were not hydrolyzed by nitrous acid elimination treatment.
T5 516-596 Sentence denotes The 35S-proteoglycans were not hydrolyzed by nitrous acid elimination treatment.
T6 597-711 Sentence denotes The chondroitinase ABC-generated disaccharides were analyzed by aminocyano high performance liquid chromatography.
T6 597-711 Sentence denotes The chondroitinase ABC-generated disaccharides were analyzed by aminocyano high performance liquid chromatography.
T7 712-989 Sentence denotes 35S-Disaccharides eluted in a major peak at a retention time of 8.1 min, corresponding to the disaccharide of chondroitin 4-sulfate proteoglycan (delta Di-4S), and a second peak at 12 min, corresponding to the disaccharide of chondroitin sulfate D proteoglycan (delta Di-diSD).
T7 712-989 Sentence denotes 35S-Disaccharides eluted in a major peak at a retention time of 8.1 min, corresponding to the disaccharide of chondroitin 4-sulfate proteoglycan (delta Di-4S), and a second peak at 12 min, corresponding to the disaccharide of chondroitin sulfate D proteoglycan (delta Di-diSD).
T8 990-1244 Sentence denotes Further treatment with chondro-4-sulfatase did not affect the retention time of the disaccharide corresponding to delta Di-diSD whereas this peak disappeared after the digested proteoglycan was treated either by chondro-6-sulfatase or by both sulfatases.
T8 990-1244 Sentence denotes Further treatment with chondro-4-sulfatase did not affect the retention time of the disaccharide corresponding to delta Di-diSD whereas this peak disappeared after the digested proteoglycan was treated either by chondro-6-sulfatase or by both sulfatases.
T9 1245-1314 Sentence denotes Therefore, this disaccharide was identified as chondroitin sulfate D.
T9 1245-1314 Sentence denotes Therefore, this disaccharide was identified as chondroitin sulfate D.
T10 1315-1508 Sentence denotes Quantification of the radiolabeled disaccharides showed that delta Di-diSD contributed 20% +/- 2% (n = 3) of the total sulfated disaccharides of the chondroitin sulfate of these cultured cells.
T10 1315-1508 Sentence denotes Quantification of the radiolabeled disaccharides showed that delta Di-diSD contributed 20% +/- 2% (n = 3) of the total sulfated disaccharides of the chondroitin sulfate of these cultured cells.
T11 1509-1784 Sentence denotes The role of fibroblasts in inducing the shift of chondroitin sulfate D into heparin proteoglycan in these mast cells was also investigated by using three types of monolayers: mouse embryonic skin fibroblasts (MESF), rat embryonic skin fibroblasts (RESF), and 3T3 fibroblasts.
T11 1509-1784 Sentence denotes The role of fibroblasts in inducing the shift of chondroitin sulfate D into heparin proteoglycan in these mast cells was also investigated by using three types of monolayers: mouse embryonic skin fibroblasts (MESF), rat embryonic skin fibroblasts (RESF), and 3T3 fibroblasts.
T12 1785-2017 Sentence denotes 35S-Proteoglycans that were extracted from the lymph node-derived mast cells cultured for 30 days on MESF and on 3T3 fibroblast monolayers were 93% +/- 4% and 30% +/- 7% (n = 3) susceptible to nitrous acid elimination, respectively.
T12 1785-2017 Sentence denotes 35S-Proteoglycans that were extracted from the lymph node-derived mast cells cultured for 30 days on MESF and on 3T3 fibroblast monolayers were 93% +/- 4% and 30% +/- 7% (n = 3) susceptible to nitrous acid elimination, respectively.
T13 2018-2131 Sentence denotes No degradation by nitrous acid was observed in 35S-proteoglycans extracted from cells cultured on RESF monolayer.
T13 2018-2131 Sentence denotes No degradation by nitrous acid was observed in 35S-proteoglycans extracted from cells cultured on RESF monolayer.
T14 2132-2375 Sentence denotes Since the MESF was found to be the most potent monolayer in the induction of heparin synthesis, the kinetics of changes in the synthesis of proteoglycan types were determined in lymph node-derived mast cells cultured on MESF for up to 30 days.
T14 2132-2375 Sentence denotes Since the MESF was found to be the most potent monolayer in the induction of heparin synthesis, the kinetics of changes in the synthesis of proteoglycan types were determined in lymph node-derived mast cells cultured on MESF for up to 30 days.
T15 2376-2538 Sentence denotes It was found that the synthesis of chondroitin sulfate gradually declined whereas that of heparin starting between 4 and 7 days after plating gradually increased.
T15 2376-2538 Sentence denotes It was found that the synthesis of chondroitin sulfate gradually declined whereas that of heparin starting between 4 and 7 days after plating gradually increased.
T16 2539-2604 Sentence denotes From the 17th day on, only the synthesis of heparin was detected.
T16 2539-2604 Sentence denotes From the 17th day on, only the synthesis of heparin was detected.