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PubMed:21147282 JSONTXT 41 Projects

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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-45 Sentence denotes The sheep milk fat globule membrane proteome.
T1 0-45 Sentence denotes The sheep milk fat globule membrane proteome.
TextSentencer_T2 46-290 Sentence denotes Milk fat globule membranes (MFGM) are three-layered structures that enclose fat droplets, and are composed by an internal monolayer of endoplasmic reticulum origin, surrounded by a bilayer derived from the apical membrane of the lactating cell.
T2 46-290 Sentence denotes Milk fat globule membranes (MFGM) are three-layered structures that enclose fat droplets, and are composed by an internal monolayer of endoplasmic reticulum origin, surrounded by a bilayer derived from the apical membrane of the lactating cell.
TextSentencer_T3 291-523 Sentence denotes In this work, an optimized protein extraction method was applied to sheep MFGM, and extracts were subjected to SDS-PAGE separation followed by shotgun LC tandem mass spectrometry (GeLC-MS/MS) for identification and characterization.
T3 291-523 Sentence denotes In this work, an optimized protein extraction method was applied to sheep MFGM, and extracts were subjected to SDS-PAGE separation followed by shotgun LC tandem mass spectrometry (GeLC-MS/MS) for identification and characterization.
TextSentencer_T4 524-590 Sentence denotes In total, 140 unique sheep MFGM proteins (MFGMPs) were identified.
T4 524-590 Sentence denotes In total, 140 unique sheep MFGM proteins (MFGMPs) were identified.
TextSentencer_T5 591-705 Sentence denotes All protein identification data were subjected to Gene Ontology (GO) classification for localization and function.
T5 591-705 Sentence denotes All protein identification data were subjected to Gene Ontology (GO) classification for localization and function.
TextSentencer_T6 706-887 Sentence denotes Moreover, the relative abundance of all identified MFGMPs was estimated by means of the normalized spectral abundance factor (NSAF) approach, and GO abundance classes were obtained.
T6 706-887 Sentence denotes Moreover, the relative abundance of all identified MFGMPs was estimated by means of the normalized spectral abundance factor (NSAF) approach, and GO abundance classes were obtained.
TextSentencer_T7 888-1118 Sentence denotes The data gathered in this work provide a detailed picture of the proteome expressed in healthy sheep MFGs, and lay the foundations for future studies on sheep lactation physiology and on its alterations in pathological conditions.
T7 888-1118 Sentence denotes The data gathered in this work provide a detailed picture of the proteome expressed in healthy sheep MFGs, and lay the foundations for future studies on sheep lactation physiology and on its alterations in pathological conditions.