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PubMed:20798114 JSONTXT 54 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-102 Sentence denotes Production and characterization of a monomeric form and a single-site form of Aleuria aurantia lectin.
T1 0-102 Sentence denotes Production and characterization of a monomeric form and a single-site form of Aleuria aurantia lectin.
T1 0-102 Sentence denotes Production and characterization of a monomeric form and a single-site form of Aleuria aurantia lectin.
TextSentencer_T2 103-195 Sentence denotes Lectins have widely been used in structural and functional studies of complex carbohydrates.
T2 103-195 Sentence denotes Lectins have widely been used in structural and functional studies of complex carbohydrates.
T2 103-195 Sentence denotes Lectins have widely been used in structural and functional studies of complex carbohydrates.
TextSentencer_T3 196-298 Sentence denotes They usually bind carbohydrates with relatively low affinity, but compensate for this by multivalency.
T3 196-298 Sentence denotes They usually bind carbohydrates with relatively low affinity, but compensate for this by multivalency.
T3 196-298 Sentence denotes They usually bind carbohydrates with relatively low affinity, but compensate for this by multivalency.
TextSentencer_T4 299-501 Sentence denotes This multivalent nature of lectins can sometimes produce unwanted reactions such as agglutination or precipitation of target glycoproteins, when using them in different biological and analytical assays.
T4 299-501 Sentence denotes This multivalent nature of lectins can sometimes produce unwanted reactions such as agglutination or precipitation of target glycoproteins, when using them in different biological and analytical assays.
T4 299-501 Sentence denotes This multivalent nature of lectins can sometimes produce unwanted reactions such as agglutination or precipitation of target glycoproteins, when using them in different biological and analytical assays.
TextSentencer_T5 502-583 Sentence denotes The mushroom lectin Aleuria aurantia binds to fucose-containing oligosaccharides.
T5 502-583 Sentence denotes The mushroom lectin Aleuria aurantia binds to fucose-containing oligosaccharides.
T5 502-583 Sentence denotes The mushroom lectin Aleuria aurantia binds to fucose-containing oligosaccharides.
TextSentencer_T6 584-682 Sentence denotes It is composed of two identical subunits, and each subunit contains five binding sites for fucose.
T6 584-682 Sentence denotes It is composed of two identical subunits, and each subunit contains five binding sites for fucose.
T6 584-682 Sentence denotes It is composed of two identical subunits, and each subunit contains five binding sites for fucose.
TextSentencer_T7 683-773 Sentence denotes In this study, two forms of recombinant AAL were produced using site-directed mutagenesis.
T7 683-773 Sentence denotes In this study, two forms of recombinant AAL were produced using site-directed mutagenesis.
T7 683-773 Sentence denotes In this study, two forms of recombinant AAL were produced using site-directed mutagenesis.
TextSentencer_T8 774-985 Sentence denotes A monomeric form of AAL was produced by exchanging Tyr6 with Arg6, and a single-site fragment of AAL was produced by insertion of an NdeI restriction enzyme cleavage site and a stop codon in the coding sequence.
T8 774-985 Sentence denotes A monomeric form of AAL was produced by exchanging Tyr6 with Arg6, and a single-site fragment of AAL was produced by insertion of an NdeI restriction enzyme cleavage site and a stop codon in the coding sequence.
T8 774-985 Sentence denotes A monomeric form of AAL was produced by exchanging Tyr6 with Arg6, and a single-site fragment of AAL was produced by insertion of an NdeI restriction enzyme cleavage site and a stop codon in the coding sequence.
TextSentencer_T9 986-1098 Sentence denotes The AAL forms were expressed as His-tagged proteins in Escherichia coli and purified by affinity chromatography.
T9 986-1098 Sentence denotes The AAL forms were expressed as His-tagged proteins in Escherichia coli and purified by affinity chromatography.
T9 986-1098 Sentence denotes The AAL forms were expressed as His-tagged proteins in Escherichia coli and purified by affinity chromatography.
TextSentencer_T10 1099-1260 Sentence denotes Binding properties of the two AAL forms were performed using surface plasmon resonance, enzyme-linked lectin assay analyses and isothermal titration calorimetry.
T10 1099-1260 Sentence denotes Binding properties of the two AAL forms were performed using surface plasmon resonance, enzyme-linked lectin assay analyses and isothermal titration calorimetry.
T10 1099-1260 Sentence denotes Binding properties of the two AAL forms were performed using surface plasmon resonance, enzyme-linked lectin assay analyses and isothermal titration calorimetry.
TextSentencer_T11 1261-1391 Sentence denotes Both the monomeric AAL (mAAL) and the single-site AAL (S2-AAL) forms retained their capacity to bind fucosylated oligosaccharides.
T11 1261-1391 Sentence denotes Both the monomeric AAL (mAAL) and the single-site AAL (S2-AAL) forms retained their capacity to bind fucosylated oligosaccharides.
T11 1261-1391 Sentence denotes Both the monomeric AAL (mAAL) and the single-site AAL (S2-AAL) forms retained their capacity to bind fucosylated oligosaccharides.
TextSentencer_T12 1392-1611 Sentence denotes However, both constructs exhibited properties that differed from the intact recombinant AAL (rAAL). mAAL showed similar binding affinities to fucosylated oligosaccharides as rAAL, but had less hemagglutinating capacity.
T12 1392-1611 Sentence denotes However, both constructs exhibited properties that differed from the intact recombinant AAL (rAAL). mAAL showed similar binding affinities to fucosylated oligosaccharides as rAAL, but had less hemagglutinating capacity.
T12 1392-1611 Sentence denotes However, both constructs exhibited properties that differed from the intact recombinant AAL (rAAL). mAAL showed similar binding affinities to fucosylated oligosaccharides as rAAL, but had less hemagglutinating capacity.
TextSentencer_T13 1612-1774 Sentence denotes S2-AAL showed a lower binding affinity to fucosylated oligosaccharides and, in contrast to rAAL and mAAL, S2-AAL did not bind to sialylated fuco-oligosaccharides.
T13 1612-1774 Sentence denotes S2-AAL showed a lower binding affinity to fucosylated oligosaccharides and, in contrast to rAAL and mAAL, S2-AAL did not bind to sialylated fuco-oligosaccharides.
T13 1612-1774 Sentence denotes S2-AAL showed a lower binding affinity to fucosylated oligosaccharides and, in contrast to rAAL and mAAL, S2-AAL did not bind to sialylated fuco-oligosaccharides.
TextSentencer_T14 1775-1960 Sentence denotes The study shows that molecular engineering is a highly useful tool for producing lectins with more defined properties such as decreased valency and defined specificities and affinities.
T14 1775-1960 Sentence denotes The study shows that molecular engineering is a highly useful tool for producing lectins with more defined properties such as decreased valency and defined specificities and affinities.
T14 1775-1960 Sentence denotes The study shows that molecular engineering is a highly useful tool for producing lectins with more defined properties such as decreased valency and defined specificities and affinities.
TextSentencer_T15 1961-2082 Sentence denotes Thus, this approach has high potential in developing reliable diagnostic and biological assays for carbohydrate analysis.
T15 1961-2082 Sentence denotes Thus, this approach has high potential in developing reliable diagnostic and biological assays for carbohydrate analysis.
T15 1961-2082 Sentence denotes Thus, this approach has high potential in developing reliable diagnostic and biological assays for carbohydrate analysis.