| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-124 |
Sentence |
denotes |
Requirement of calcium binding, myristoylation, and protein-protein interaction for the Copine BON1 function in Arabidopsis. |
| T2 |
125-232 |
Sentence |
denotes |
Copines are highly conserved proteins with lipid-binding activities found in animals, plants, and protists. |
| T3 |
233-364 |
Sentence |
denotes |
They contain two calcium-dependent phospholipid binding C2 domains at the amino terminus and a VWA domain at the carboxyl terminus. |
| T4 |
365-501 |
Sentence |
denotes |
The biological roles of most copines are not understood and the biochemical properties required for their functions are largely unknown. |
| T5 |
502-600 |
Sentence |
denotes |
The Arabidopsis copine gene BON1/CPN1 is a negative regulator of cell death and defense responses. |
| T6 |
601-687 |
Sentence |
denotes |
Here we probed the potential biochemical activities of BON1 through mutagenic studies. |
| T7 |
688-817 |
Sentence |
denotes |
We found that mutations of aspartates in the C2 domains did not alter plasma membrane localization but compromised BON1 activity. |
| T8 |
818-944 |
Sentence |
denotes |
Mutation at putative myristoylation residue glycine 2 altered plasma membrane localization of BON1 and rendered BON1 inactive. |
| T9 |
945-1036 |
Sentence |
denotes |
Mass spectrometry analysis of BON1 further suggests that the N-peptide of BON1 is modified. |
| T10 |
1037-1157 |
Sentence |
denotes |
Furthermore, mutations that affect the interaction between BON1 and its functional partner BAP1 abolished BON1 function. |
| T11 |
1158-1378 |
Sentence |
denotes |
This analysis reveals an unanticipated regulation of copine protein localization and function by calcium and lipid modification and suggests an important role in protein-protein interaction for the VWA domain of copines. |
