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PubMed:20427278 JSONTXT 24 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-139 Sentence denotes The novel membrane protein TMEM59 modulates complex glycosylation, cell surface expression, and secretion of the amyloid precursor protein.
T2 140-344 Sentence denotes Ectodomain shedding of the amyloid precursor protein (APP) by the two proteases alpha- and beta-secretase is a key regulatory event in the generation of the Alzheimer disease amyloid beta peptide (Abeta).
T3 345-450 Sentence denotes At present, little is known about the cellular mechanisms that control APP shedding and Abeta generation.
T4 451-558 Sentence denotes Here, we identified a novel protein, transmembrane protein 59 (TMEM59), as a new modulator of APP shedding.
T5 559-632 Sentence denotes TMEM59 was found to be a ubiquitously expressed, Golgi-localized protein.
T6 633-719 Sentence denotes TMEM59 transfection inhibited complex N- and O-glycosylation of APP in cultured cells.
T7 720-940 Sentence denotes Additionally, TMEM59 induced APP retention in the Golgi and inhibited Abeta generation as well as APP cleavage by alpha- and beta-secretase cleavage, which occur at the plasma membrane and in the endosomes, respectively.
T8 941-1088 Sentence denotes Moreover, TMEM59 inhibited the complex N-glycosylation of the prion protein, suggesting a more general modulation of Golgi glycosylation reactions.
T9 1089-1296 Sentence denotes Importantly, TMEM59 did not affect the secretion of soluble proteins or the alpha-secretase like shedding of tumor necrosis factor alpha, demonstrating that TMEM59 did not disturb the general Golgi function.
T10 1297-1473 Sentence denotes The phenotype of TMEM59 transfection on APP glycosylation and shedding was similar to the one observed in cells lacking conserved oligomeric Golgi (COG) proteins COG1 and COG2.
T11 1474-1569 Sentence denotes Both proteins are required for normal localization and activity of Golgi glycosylation enzymes.
T12 1570-1823 Sentence denotes In summary, this study shows that TMEM59 expression modulates complex N- and O-glycosylation and suggests that TMEM59 affects APP shedding by reducing access of APP to the cellular compartments, where it is normally cleaved by alpha- and beta-secretase.