> top > projects > sentences > docs > PubMed:20139089 > annotations
sentences Home  

PubMed:20139089 JSONTXT 23 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-139 Sentence denotes Molecular interaction and functional regulation of ClC-3 by Ca2+/calmodulin-dependent protein kinase II (CaMKII) in human malignant glioma.
T2 140-225 Sentence denotes Glioblastoma multiforme is the most common and lethal primary brain cancer in adults.
T3 226-327 Sentence denotes Tumor cells diffusely infiltrate the brain making focal surgical and radiation treatment challenging.
T4 328-463 Sentence denotes The invasion of glioma cells into normal brain is facilitated by the activity of ion channels aiding dynamic regulation of cell volume.
T5 464-565 Sentence denotes Recent studies have specifically implicated ClC-3, a voltage-gated chloride channel, in this process.
T6 566-653 Sentence denotes However, the interaction between ClC-3 activity and cell movement is poorly understood.
T7 654-866 Sentence denotes Here, we demonstrate that ClC-3 is highly expressed on the plasma membrane of human glioma cells where its activity is regulated through phosphorylation via Ca(2+)/calmodulin-dependent protein kinase II (CaMKII).
T8 867-1074 Sentence denotes Intracellular infusion of autoactivated CaMKII via patch pipette enhanced chloride currents 3-fold, and this regulation was inhibited by autocamtide-2 related inhibitory peptide, a CaMKII-specific inhibitor.
T9 1075-1240 Sentence denotes CaMKII modulation of chloride currents was also lost upon stable small hairpin RNA knockdown of ClC-3 channels indicating a specific interaction of ClC-3 and CaMKII.
T10 1241-1362 Sentence denotes In ClC-3-expressing cells, inhibition of CaMKII reduced glioma invasion to the same extent as direct inhibition of ClC-3.
T11 1363-1525 Sentence denotes The importance of the molecular interaction of ClC-3 and CaMKII is further supported by our finding that CaMKII co-localizes and co-immunoprecipitates with ClC-3.
T12 1526-1639 Sentence denotes ClC-3 and CaMKII also co-immunoprecipitate in tissue biopsies from patients diagnosed with grade IV glioblastoma.
T13 1640-1729 Sentence denotes These tumor samples show 10-fold higher ClC-3 protein expression than nonmalignant brain.
T14 1730-1941 Sentence denotes These data suggest that CaMKII is a molecular link translating intracellular calcium changes, which are intrinsically associated with glioma migration, to changes in ClC-3 conductance required for cell movement.