| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-221 |
Sentence |
denotes |
Regulation of SIV mac 239 basal long terminal repeat activity and viral replication in macrophages: functional roles of two CCAAT/enhancer-binding protein beta sites in activation and interferon beta-mediated suppression. |
| T2 |
222-487 |
Sentence |
denotes |
CCAAT/enhancer-binding protein (C/EBP) beta and C/EBP sites in the HIV-1 long terminal repeat (LTR) are crucial for HIV-1 replication in monocyte/macrophages and for the ability of interferon beta (IFN beta) to inhibit ongoing active HIV replication in these cells. |
| T3 |
488-675 |
Sentence |
denotes |
This IFN beta-mediated down-regulation involves induction of the truncated, dominant-negative isoform of C/EBP beta referred to as liver-enriched transcriptional inhibitory protein (LIP). |
| T4 |
676-1023 |
Sentence |
denotes |
Although binding of the C/EBP beta isoform to C/EBP sites in the simian immunodeficiency virus (SIV) LTR has previously been examined, the importance of these sites in core promoter-mediated transcription, virus replication, IFN beta-mediated regulation, and the relative binding of the two isoforms (C/EBP beta and LIP) has not been investigated. |
| T5 |
1024-1241 |
Sentence |
denotes |
Here, we specifically examine two C/EBP sites, JC1 (-100 bp) and DS1 (+134 bp), located within the minimal region of the SIV LTR, required for core promoter-mediated transcription and virus replication in macrophages. |
| T6 |
1242-1440 |
Sentence |
denotes |
Our studies revealed that the JC1 but not DS1 C/EBP site is important for basal level transcription, whereas the DS1 C/EBP site is imperative for productive virus replication in primary macrophages. |
| T7 |
1441-1598 |
Sentence |
denotes |
In contrast, either JC1 or DS1 C/EBP site is sufficient to mediate IFN beta-induced down-regulation of SIV LTR activity and virus replication in these cells. |
| T8 |
1599-1704 |
Sentence |
denotes |
We also characterized the differential binding properties of C/EBP beta and LIP to the JC1 and DS1 sites. |
| T9 |
1705-1940 |
Sentence |
denotes |
In conjunction with previous studies from our laboratory, we demonstrate the importance of these sites in virus gene expression, and we propose a model for their role in establishing latency and persistence in macrophages in the brain. |
