| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-109 |
Sentence |
denotes |
Establishment of a real-time analytical method for free oligosaccharide transport from the ER to the cytosol. |
| T1 |
0-109 |
Sentence |
denotes |
Establishment of a real-time analytical method for free oligosaccharide transport from the ER to the cytosol. |
| T1 |
0-109 |
Sentence |
denotes |
Establishment of a real-time analytical method for free oligosaccharide transport from the ER to the cytosol. |
| TextSentencer_T2 |
110-261 |
Sentence |
denotes |
During N-glycosylation of proteins, significant amounts of free unconjugated glycans are also generated in the lumen of the endoplasmic reticulum (ER). |
| T2 |
110-261 |
Sentence |
denotes |
During N-glycosylation of proteins, significant amounts of free unconjugated glycans are also generated in the lumen of the endoplasmic reticulum (ER). |
| T2 |
110-261 |
Sentence |
denotes |
During N-glycosylation of proteins, significant amounts of free unconjugated glycans are also generated in the lumen of the endoplasmic reticulum (ER). |
| TextSentencer_T3 |
262-394 |
Sentence |
denotes |
These ER-derived free glycans are translocated into the cytosol by a putative transporter on the ER membrane for further processing. |
| T3 |
262-394 |
Sentence |
denotes |
These ER-derived free glycans are translocated into the cytosol by a putative transporter on the ER membrane for further processing. |
| T3 |
262-394 |
Sentence |
denotes |
These ER-derived free glycans are translocated into the cytosol by a putative transporter on the ER membrane for further processing. |
| TextSentencer_T4 |
395-469 |
Sentence |
denotes |
However, the molecular nature of the transporter remains to be determined. |
| T4 |
395-469 |
Sentence |
denotes |
However, the molecular nature of the transporter remains to be determined. |
| T4 |
395-469 |
Sentence |
denotes |
However, the molecular nature of the transporter remains to be determined. |
| TextSentencer_T5 |
470-652 |
Sentence |
denotes |
Here, we report the establishment of a novel assay method for free oligosaccharide transport from the ER lumen using chemically synthesized fluorescence-labeled N-glycan derivatives. |
| T5 |
470-652 |
Sentence |
denotes |
Here, we report the establishment of a novel assay method for free oligosaccharide transport from the ER lumen using chemically synthesized fluorescence-labeled N-glycan derivatives. |
| T5 |
470-652 |
Sentence |
denotes |
Here, we report the establishment of a novel assay method for free oligosaccharide transport from the ER lumen using chemically synthesized fluorescence-labeled N-glycan derivatives. |
| TextSentencer_T6 |
653-860 |
Sentence |
denotes |
In this method, fluorescence-labeled glycan substrates were encapsulated inside mouse liver microsomes, followed by incubation with the cytosol and a fluorescence-quenching agent (anti-fluorophore antibody). |
| T6 |
653-860 |
Sentence |
denotes |
In this method, fluorescence-labeled glycan substrates were encapsulated inside mouse liver microsomes, followed by incubation with the cytosol and a fluorescence-quenching agent (anti-fluorophore antibody). |
| T6 |
653-860 |
Sentence |
denotes |
In this method, fluorescence-labeled glycan substrates were encapsulated inside mouse liver microsomes, followed by incubation with the cytosol and a fluorescence-quenching agent (anti-fluorophore antibody). |
| TextSentencer_T7 |
861-968 |
Sentence |
denotes |
The rate of substrate efflux was then monitored in real time by the decrease in the fluorescence intensity. |
| T7 |
861-968 |
Sentence |
denotes |
The rate of substrate efflux was then monitored in real time by the decrease in the fluorescence intensity. |
| T7 |
861-968 |
Sentence |
denotes |
The rate of substrate efflux was then monitored in real time by the decrease in the fluorescence intensity. |
| TextSentencer_T8 |
969-1121 |
Sentence |
denotes |
The present data clearly demonstrated that the oligosaccharide transport activity under the current assay conditions was both ATP and cytosol dependent. |
| T8 |
969-1121 |
Sentence |
denotes |
The present data clearly demonstrated that the oligosaccharide transport activity under the current assay conditions was both ATP and cytosol dependent. |
| T8 |
969-1121 |
Sentence |
denotes |
The present data clearly demonstrated that the oligosaccharide transport activity under the current assay conditions was both ATP and cytosol dependent. |
| TextSentencer_T9 |
1122-1280 |
Sentence |
denotes |
The transporter activity was also found to be glycan structure specific because free glucosylated glycans were unable to be transported out of the microsomes. |
| T9 |
1122-1280 |
Sentence |
denotes |
The transporter activity was also found to be glycan structure specific because free glucosylated glycans were unable to be transported out of the microsomes. |
| T9 |
1122-1280 |
Sentence |
denotes |
The transporter activity was also found to be glycan structure specific because free glucosylated glycans were unable to be transported out of the microsomes. |
| TextSentencer_T10 |
1281-1384 |
Sentence |
denotes |
This new assay method will be a useful tool for identifying the transporter protein on the ER membrane. |
| T10 |
1281-1384 |
Sentence |
denotes |
This new assay method will be a useful tool for identifying the transporter protein on the ER membrane. |
| T10 |
1281-1384 |
Sentence |
denotes |
This new assay method will be a useful tool for identifying the transporter protein on the ER membrane. |