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PubMed:19494346 JSONTXT 50 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-109 Sentence denotes Establishment of a real-time analytical method for free oligosaccharide transport from the ER to the cytosol.
T1 0-109 Sentence denotes Establishment of a real-time analytical method for free oligosaccharide transport from the ER to the cytosol.
T1 0-109 Sentence denotes Establishment of a real-time analytical method for free oligosaccharide transport from the ER to the cytosol.
TextSentencer_T2 110-261 Sentence denotes During N-glycosylation of proteins, significant amounts of free unconjugated glycans are also generated in the lumen of the endoplasmic reticulum (ER).
T2 110-261 Sentence denotes During N-glycosylation of proteins, significant amounts of free unconjugated glycans are also generated in the lumen of the endoplasmic reticulum (ER).
T2 110-261 Sentence denotes During N-glycosylation of proteins, significant amounts of free unconjugated glycans are also generated in the lumen of the endoplasmic reticulum (ER).
TextSentencer_T3 262-394 Sentence denotes These ER-derived free glycans are translocated into the cytosol by a putative transporter on the ER membrane for further processing.
T3 262-394 Sentence denotes These ER-derived free glycans are translocated into the cytosol by a putative transporter on the ER membrane for further processing.
T3 262-394 Sentence denotes These ER-derived free glycans are translocated into the cytosol by a putative transporter on the ER membrane for further processing.
TextSentencer_T4 395-469 Sentence denotes However, the molecular nature of the transporter remains to be determined.
T4 395-469 Sentence denotes However, the molecular nature of the transporter remains to be determined.
T4 395-469 Sentence denotes However, the molecular nature of the transporter remains to be determined.
TextSentencer_T5 470-652 Sentence denotes Here, we report the establishment of a novel assay method for free oligosaccharide transport from the ER lumen using chemically synthesized fluorescence-labeled N-glycan derivatives.
T5 470-652 Sentence denotes Here, we report the establishment of a novel assay method for free oligosaccharide transport from the ER lumen using chemically synthesized fluorescence-labeled N-glycan derivatives.
T5 470-652 Sentence denotes Here, we report the establishment of a novel assay method for free oligosaccharide transport from the ER lumen using chemically synthesized fluorescence-labeled N-glycan derivatives.
TextSentencer_T6 653-860 Sentence denotes In this method, fluorescence-labeled glycan substrates were encapsulated inside mouse liver microsomes, followed by incubation with the cytosol and a fluorescence-quenching agent (anti-fluorophore antibody).
T6 653-860 Sentence denotes In this method, fluorescence-labeled glycan substrates were encapsulated inside mouse liver microsomes, followed by incubation with the cytosol and a fluorescence-quenching agent (anti-fluorophore antibody).
T6 653-860 Sentence denotes In this method, fluorescence-labeled glycan substrates were encapsulated inside mouse liver microsomes, followed by incubation with the cytosol and a fluorescence-quenching agent (anti-fluorophore antibody).
TextSentencer_T7 861-968 Sentence denotes The rate of substrate efflux was then monitored in real time by the decrease in the fluorescence intensity.
T7 861-968 Sentence denotes The rate of substrate efflux was then monitored in real time by the decrease in the fluorescence intensity.
T7 861-968 Sentence denotes The rate of substrate efflux was then monitored in real time by the decrease in the fluorescence intensity.
TextSentencer_T8 969-1121 Sentence denotes The present data clearly demonstrated that the oligosaccharide transport activity under the current assay conditions was both ATP and cytosol dependent.
T8 969-1121 Sentence denotes The present data clearly demonstrated that the oligosaccharide transport activity under the current assay conditions was both ATP and cytosol dependent.
T8 969-1121 Sentence denotes The present data clearly demonstrated that the oligosaccharide transport activity under the current assay conditions was both ATP and cytosol dependent.
TextSentencer_T9 1122-1280 Sentence denotes The transporter activity was also found to be glycan structure specific because free glucosylated glycans were unable to be transported out of the microsomes.
T9 1122-1280 Sentence denotes The transporter activity was also found to be glycan structure specific because free glucosylated glycans were unable to be transported out of the microsomes.
T9 1122-1280 Sentence denotes The transporter activity was also found to be glycan structure specific because free glucosylated glycans were unable to be transported out of the microsomes.
TextSentencer_T10 1281-1384 Sentence denotes This new assay method will be a useful tool for identifying the transporter protein on the ER membrane.
T10 1281-1384 Sentence denotes This new assay method will be a useful tool for identifying the transporter protein on the ER membrane.
T10 1281-1384 Sentence denotes This new assay method will be a useful tool for identifying the transporter protein on the ER membrane.